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血管动力学在骨骨髓的静脉直通纵向成像

Aleria Duperray-Susini¹, Thomas Guilbert², Jozsua Fodor³

¹Leukemia and Niche Dynamics laboratory, Institut Cochin, Université Paris Cité UMR-S1016, INSERM U1016, CNRS UMR8104.

Journal of visualized experiments : JoVE

|April 28, 2025

概括

这项研究引入了一种新的静脉内显微镜协议,用于对骨髓的纵向成像. 它使血管动力学和细胞相互作用的详细可视化成为可能,这对于理解组织再生和瘤发育至关重要.

科学领域:

* 在体内成像和显微镜.
* 血管生物学和瘤学
* 血液形成和组织再生

背景情况:

*固定组织的静态成像限制了对动态生物过程的理解.
*卡尔瓦里骨髓是一种动态的微环境,对血液形成至关重要.
* 肠道显微镜可以实时了解细胞和血管的行为.

研究的目的:

* 开发和验证骨骨髓的静脉纵向光显微镜协议.
* 为了使血管动力学和细胞相互作用的空间和时间检查.
* 为研究瘤发育和体内治疗反应提供一个平台.

主要方法:

* 使用转基因动物具有细胞特异标记物和活细胞追踪剂.
*采用3D打印的头固定植入物,用于稳定的纵向成像.
* 应用双光子显微镜用于高分辨率的血管床和细胞动态的可视化.

主要成果:

*成功地可视化和量化了骨髓中的血管异质性.
* 随着时间的推移,监测了血管,层状细胞和造血细胞之间的相互作用.
* 能够评估治疗效果,干细胞调动和药物局部化.

结论:

* 开发的协议提供了一个强大的工具,用于体内调查骨髓微环境.
* 提供了前所未有的洞察力,了解瘤血管生成和血液形成等动态过程.
* 促进治疗干预的研究及其对血管和细胞动态的影响.

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