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Updated: May 12, 2025

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
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通过在纳米孔中直接全长测序对微RNA进行定量和复合分析

Chenzhi Shi1,2, Donglei Yang2, Xiaowei Ma1

  • 1Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.

Journal of the American Chemical Society
|April 28, 2025
PubMed
概括

直接miR-seq是一种纳米孔测序方法,可实现全长的微RNA测序,以全面分析它们的丰度和修饰. 这一突破为复杂生物样本中的小RNA研究提供了高产量和精度.

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Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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