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多阶段分裂 (MS³) 检测提高了血清类固醇激素LC-MS分析的定量敏感性和准确性

Keke Yi¹, Wei Wan², Ziyu Qu³

¹Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, China; School of Physics Science and Engineering, Tongji University, Shanghai, 200092, China.

|April 28, 2025

概括

使用四极-线性离子陷协同质谱法 (Q-LIT) 的新方法可以准确测量人血清中超微量类固醇激素的含量. 这种多阶段碎片化 (MS3) 技术提高了灵敏度,并减少了矩阵干扰,以精确检测激素.

科学领域:

分析化学分析化学
生物化学生化学
质谱测量质量谱测量

背景情况:

对人血清中超微量类固醇激素的准确量化对于诊断各种内分泌疾病至关重要.
传统方法通常需要大量的样本准备,并且受到矩阵效应的限制,影响灵敏度和准确度.
三重四极质谱 (MS2) 的碎片化能力有限,阻碍了更深入的结构分析.

研究的目的:

建立一种新的测定方法,用于人类血清中超微量类固醇激素,使用自制四极线性离子陷协同质谱 (Q-LIT) 系统.
为了利用Q-LIT的多阶段碎片化 (MS3) 能力,增强结构特征和提高检测灵敏度.
用真实血清样本验证该方法在线性,检测和量化极限,回收和精度方面的性能.

主要方法:

开发一个具有多阶段碎片化 (MS3) 功能的四极线性离子陷并列质谱 (Q-LIT) 系统.
使用特定的MS3转换量化五种类固醇激素 (皮松,4-androstenedione,11-deoxycortisol,21-deoxycortisol和17α-hydroxyprogesterone) 的量化.
评估方法性能,包括线性 (R2 ≥0.99),检测极限 (<0.06 ng/mL) 和量化 (<0.20 ng/mL),恢复 (86.56%-123.14%) 和相对标准偏差 (RSD ≤13.57%).

主要成果:

该MS3方法表现出高灵敏度和有效降低噪声,使得可以准确检测类固醇激素,而无需广泛的衍生或样本准备.
与MS2相比,MS3显著减轻了矩阵干扰,导致了更可靠的定量结果.
该方法准确而灵敏地测量了22个真人血清样本,证实了其实际适用性.

结论:

已建立的基于Q-LIT的多阶段碎片化方法为人类血清中超微量类固醇激素的确定提供了灵敏而准确的方法.
MS3功能提供了优越的结构特征,克服了与矩阵效应相关的局限性,使其适合复杂的生物样本.
这种技术对于需要检测具有严重矩阵效应的类固醇激素和相关异构体的分化应用具有重大潜力.

关键词:

在 LCMS 中使用.线性离子陷是一种线性离子陷.多阶段的碎片化是多阶段的碎片化.血清的血清是什么意思类固醇激素激素是一种类固醇.

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