通过NMD介导的后转录调节微调NLR-WRKY调节模块以调节细菌防御反应
Zeeshan Nasim1, Nouroz Karim1, Ikram Blilou2
1Department of Molecular Life Sciences, Korea University, Seoul 02841, Republic of Korea.
概括
无意中介的mRNA衰变 (NMD) 调节WRKY基因,影响植物免疫力. 然后,WRKY转录因子直接控制NLR基因,形成反循环以增强病原体防御.
科学领域:
- 植物分子生物学 植物分子生物学
- 转录组的完整性 转录组的完整性
- 植物免疫力 植物免疫力
背景情况:
- 无意中介的mRNA衰变 (NMD) 是维护转录组完整性的关键监控系统.
- NMD调节核酸结合,富含白素的重复 (NLR) 基因,影响植物生长并防止自身免疫.
- 对于NMD对WRKY基因的调节及其与NLRs的相互作用的了解很少.
研究的目的:
- 为了调查NMD是否直接调节WRKY基因.
- 阐明NMD调节的WRKY基因和NLR基因之间的反机制.
- 了解这种监管网络在植物对病原体防御中的作用.
主要方法:
- 在不同温度下对缺乏NMD的植物中WRKY基因表达的分析.
- 转录组分析以确定NMD调节的WRKY基因.
- ChIP-seq和DAP-seq用于评估WRKY与NLR基因促进体的结合.
主要成果:
- NMD直接调节WRKY基因的一个子集 (WRKY15,18,25,33,46,60,70),特别是在16°C时.
- 这些WRKY基因是由植物中的细菌感染和玉米和大米中的同类产生的.
- WRKY转录因子直接与NLR基因的促进子区域结合,表明其具有调节作用.
结论:
- NMD机器调节WRKY基因以控制基底防御水平.
- WRKY转录因子直接调节NLR基因,建立一个积极的反循环.
- 这种调节循环优化了植物对病原体入侵的反应,特别是以温度依赖的方式.
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