黄金纳米颗粒对Saccharomyces cerevisiae的铁死类似的死亡诱导
Min Seok Kwun1, Dong Gun Lee1,2
1School of Life Science, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, Republic of Korea.
Journal of microbiology and biotechnology
|April 28, 2025
概括
黄金纳米颗粒 (AuNPs) 诱导酵母体的铁样细胞死亡,通过导致铁过载和谷氨耗尽. 这种氧化应激导致细胞死亡,突出了AuNPs.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 微生物学 微生物学
背景情况:
- 铁死是一种受调节的细胞死亡 (RCD) 形式,在癌症中显示出治疗潜力.
- 微生物中铁灭的机制尚不清楚.
- 黄金纳米粒子 (AuNPs) 可以诱导细胞死亡,包括铁亡,用抗生素.
研究的目的:
- 调查AuNPs是否会在真核微生物*Saccharomyces cerevisiae*中诱导类似铁亡的细胞死亡.
- 为了阐明酵母中AuNP诱导的细胞死亡的分子机制.
主要方法:
- 在接受AuNPs治疗的*S. cerevisiae*中评估了细胞活力降低.
- 测量了细胞内铁水平,谷氨 (GSH) 枯竭和谷氨过氧化酶 (GPx) 活性.
- 量化反应性氧物种 (ROS) 和脂质过氧化.
- 通过膜潜力和密度分析线粒体功能障碍.
- 评估了DNA损伤标记物和metacaspase激活.
- 用了Liperfluo,一种特定于脂质ROS的探针,进行确认.
主要成果:
- AuNPs显著降低了*S. cerevisiae*的生存能力.
- 观察到铁过载,GSH耗尽和GPx无活化,这是铁亡的特征.
- 检测到ROS的增加,脂质过氧化和DNA损伤 (8-氧化瓜诺辛).
- 确认了线粒体功能障碍,包括膜潜能过极化.
- 铁性死亡缺乏染色体凝聚,DNA碎片化和元酶激活.
- 利珀果 (Liperfluo) 证实了脂质ROS的积累.
结论:
- 在*Saccharomyces cerevisiae*中,AuNP会诱导类似铁亡的细胞死亡.
- 这个过程涉及铁过载,GSH耗尽,ROS积累和脂质过氧化.
- AuNPs显示出作为抗真菌剂的潜力.
- 这些发现有助于我们更好地了解真核微生物中的铁亡.
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