高性能薄层染色学,使用含有SDS的化剂,辅助UV和拉曼光谱
Beata Polak1, Adrianna Kryska2, Kamila Jaglińska3
1Department of Physical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093, Lublin, Poland. beata.polak@umlub.pl.
Scientific reports
|April 28, 2025
概括
这项研究使用二硫酸盐 (SDS) 修改了凝板,以加强药物分离. 拉曼光谱和薄层染色学 (TLC) 用于分析神经退行性药物,改进定量分析和制药应用.
科学领域:
- 分析化学 分析化学
- 染色体学 染色体学 是一种染色学.
- 频谱学是一种光谱学.
背景情况:
- 非极性凝板通常用于染色学.
- 二硫酸盐 (SDS) 是一种表面活性剂,在修改色谱静止相方面具有潜在的应用.
- 拉曼光谱为分子相互作用和结构变化提供了洞察力.
研究的目的:
- 为了研究使用水性有机溶剂和SDS修改凝板的方法.
- 探索拉曼光谱在分析SDS修饰 Sorbents 和药物-SDS复合物的应用.
- 开发和优化薄层染色学 (TLC) 方法,用于分离和定量分析神经退行性药物,使用SDS修改的RP-18 W板.
主要方法:
- 在水有机溶剂中使用二甲基硫酸盐 (SDS) 修改RP-18W凝板.
- 使用拉曼光谱分析吸附剂修饰和碳胺-SDS复合物的分析.
- 薄层染色学 (TLC) 用于分离神经退行性药物 (硫比里德,奥兰扎,卡巴马泽,特拉佐,克洛米普拉,普里迪诺).
- 使用导电法和光谱法确定SDS的临界胞度 (CMC).
- 优化移动相组合和评价色谱参数 (尾巴系数,不对称系数,分离效率).
- 使用LOD和LOQ的定量分析和对药物制剂的应用.
主要成果:
- 拉曼光谱学证实了SDS修改后的凝吸附剂的变化,并提供了对碳胺-SDS复合体形成的见解.
- 开发的TLC方法允许分离含有六种神经退行性药物的混合物.
- 确定了SDS的临界菌根度,证实了菌根形成.
- 对于大多数化合物,色谱参数如尾部和不对称因素得到了改进,其值接近1.0.0.
- 测量了分离效率,理论板的高度从39到73微米不等.
- 优化方法对定量分析表现出良好的灵敏度,LOD值在0.22~1.67μg/spot之间,LOQ值在0.66~5.07μg/spot之间.
- 该方法已成功应用于药品制剂中药物的定量分析.
结论:
- 用SDS修改RP-18 W TLC板是一种可行的策略,可以增强神经退行性药物的分离.
- 结合使用TLC,拉曼光谱和导电法,为分析色谱系统提供了一种全面的方法.
- 与现有方法相比,这种新的方法为某些化合物提供了改进的或可比的分析性能.
- 开发的方法为制药配方中药物的定量分析开辟了新的途径.
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