源自Streptococcus mutans的细胞外囊泡通过tRNA载荷促进皮肤伤口愈合
Su Young Oh1, Dong Yeon Kim1, Kah Young Lee1
1Department of Microbiology and Immunology, School of Dentistry, Kyungpook National University, Daegu, South Korea.
Journal of nanobiotechnology
|April 28, 2025
概括
来自口腔细菌的细胞外囊泡 (EVs),特别是Streptococcus mutans EVs,促进伤口愈合. 它们的tRNA载荷激活了Toll-like受体3 (TLR3),这表明对不愈合的皮肤伤口有治疗潜力.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 口腔是各种各样的细菌的宿主,包括链球菌种.
- 快速的口腔伤口愈合机制尚未完全理解.
- 这项研究探讨了Streptococcus mutans细胞外囊泡 (Sm EVs) 在伤口愈合中的作用.
研究的目的:
- 研究Sm EVs对口腔粘膜器官和小鼠皮肤伤口愈合的影响.
- 在Sm EVs中识别影响伤口愈合的RNA载荷.
- 确定托尔类受体3 (TLR3) 在Sm EV介导的伤口愈合过程中的作用.
主要方法:
- 用口腔粘膜器官和小鼠皮肤模型来评估伤口愈合.
- RNA测序确定了Sm EVs中丰富的RNA序列.
- 合成的tRNA寡合体被封装在大肠杆菌EVs (Ec EVs) 中进行体内测试.
- 在TLR3淘汰赛 (KO) 小鼠中评估了伤口愈合.
主要成果:
- Sm EVs显著增强了口腔粘膜中的细胞增殖,迁移和焦点粘附复合体的形成.
- 在小鼠背部皮肤中,SM EVs加速了伤口愈合.
- 细菌tRNA,特别是tRNA-Met (Oligo 1),是Sm EVs中最丰富的RNA.
- 携带Oligo 1的Ec EV模仿了Sm EV对伤口愈合的影响.
- 在TLR3KO小鼠中,Oligo1的治疗效果被消除了.
结论:
- Sm EVs,特别是它们的tRNA变体,通过TLR3依赖途径促进皮肤伤口愈合.
- 来自口腔共生细菌的EV显示为慢性,不愈合的皮肤伤口的治疗承诺.
相关概念视频
Clinical Applications of Epidermal Stem Cells
2.6K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
2.6K
Vesicular Tubular Clusters
2.3K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
With the help of motor proteins such...
2.3K
Overview of Secretory Vesicles
7.2K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
7.2K
Inflammatory Response II: Inflammatory Exudate and Tissue Repair
4.4K
The immune system's inflammatory response destroys the invading pathogen, permitting the tissue to heal. The changes during the cellular and vascular stages allow exudate formation at the site of inflammation. The inflammatory exudate released from the wound has high protein content and a specific gravity above 1.020.
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the...
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the...
4.4K
Intralumenal Vesicles and Multivesicular Bodies
3.3K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.3K
Overview of Exosomes
2.7K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.7K


