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Updated: May 14, 2025

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使用粘度敏感光生命周期探测器探索对蛋白相分离的内质网膜功能障碍.

Yu Wei1, Xiangyu Zi1, Jia Zhai1

  • 1School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, China.

Analytical chemistry
|April 29, 2025
PubMed
概括

新的探测器可视化了与退行性疾病相关的蛋白质相变. 研究人员发现离子 (Ca2+) 调节TDP-43聚合,提供了潜在的治疗见解.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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