[不同年龄段的富血小板血的再生能力]
T I Vlasova1, E P Brodovskaya1, A P Vlasov1
1Ogarev National Research Mordovian State University, Saransk, Russia.
Khirurgiia
|April 29, 2025
概括
来自年轻捐赠者的富血小板血 (PRP) 增强了纤维细胞代谢活动和活力,而来自老年捐赠者的PRP则减少了它,增加了细胞死亡. 移民活动不受捐赠者年龄的影响.
科学领域:
- 再生医学是一种再生医学.
- 细胞生物学 细胞生物学
- 皮肤病学 皮肤病学
背景情况:
- 富于血小板的血 (PRP) 用于其再生性质.
- 供体年龄对皮肤纤维细胞培养中的PRP疗效的影响尚未完全理解.
- 研究年龄调整的再生潜力对于优化PRP疗法至关重要.
研究的目的:
- 评估皮肤纤维细胞上富血小板血 (PRP) 的年龄调整后的再生潜力.
- 分析不同年龄组的捐赠者用PRP刺激的纤维细胞的增殖,迁移和代谢活动.
- 评估PRP对活性氧物种 (ROS) 生产和细胞死亡机制的影响.
主要方法:
- 从30-40岁 (第一组) 和60-70岁 (第二组) 的健康捐赠者中准备了PRP.
- 皮肤纤维细胞系 (hTERT-HDFa) 被培养并用10%的供体PRP进行治疗.
- 分析了新陈代谢活动 (MTT试验),迁移 (划痕试验),ROS释放和细胞死亡 (光显微镜).
主要成果:
- 来自老年捐赠者的PRP (60-70岁) 与年轻的捐赠者 (30-40岁) 相比,显著降低了纤维细胞代谢活性85.5%.
- 年轻的捐赠者PRP最初增加了ROS释放,然后减少,而老的捐赠者PRP显示了持续的ROS增加.
- 年轻捐赠者的PRP活细胞数量最高,而年龄较大的捐赠者的PRP增加了缩和亡.
结论:
- 来自年轻捐赠者的PRP表现出一种亲氧化作用,增强新陈代谢活动,而不会在24小时内诱导细胞死亡.
- 来自老年捐赠者的PRP导致纤维细胞培养物中的代谢活性降低,和亡增加.
- 捐赠者的年龄没有影响皮肤纤维细胞的迁移活动.
相关概念视频
Structure and Function of Platelets
877
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
877
Stem Cell Culture
5.0K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
5.0K
Formation of the Platelet Plug
2.7K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
2.7K
Stem Cell Therapy for Tissue Regeneration
4.0K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.0K
iPS Cell Differentiation
2.6K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.6K


