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相关概念视频

Chromosome Structure02:40

Chromosome Structure

22.3K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
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Replication in Prokaryotes01:32

Replication in Prokaryotes

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DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
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The Replisome03:01

The Replisome

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
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Replication in Eukaryotes01:29

Replication in Eukaryotes

12.7K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Chromosome Replication02:31

Chromosome Replication

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Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
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相关实验视频

Updated: May 9, 2025

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
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Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

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OriV-Finder:一个全面的网络服务器,用于细菌等离子体复制原始分析.

Yujie Li1, Feng Gao1,2,3

  • 1Department of Physics, School of Science, Tianjin University, Tianjin 300072, China.

Nucleic acids research
|April 29, 2025
PubMed
概括
此摘要是机器生成的。

OriV-Finder是一个新的网络服务器,可以帮助科学家找到细菌等离子体复制原始 (oriVs). 它使用复制启动蛋白 (RIP) 和保存特征来准确识别和优先考虑潜在的oriVs.

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Plasmid Stability Analysis with Open-Source Droplet Microfluidics

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Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
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Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

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科学领域:

  • 微生物学 微生物学
  • 生物信息学是一种生物信息学.
  • 分子生物学分子生物学

背景情况:

  • 细菌等离子体对于水平基因转移和适应至关重要.
  • 识别植物性复制起源 (oriVs) 对于理解等离子体维护和复制至关重要.
  • 目前用于oriV识别的方法往往耗时,缺乏系统分析.

研究的目的:

  • 开发一个全面和用户友好的Web服务器,用于快速和系统地识别细菌等离子体复制起源 (oriVs).
  • 根据复制启动蛋白 (RIP) 和保存特征,提供一个准确识别潜在 oriV 的工具.

主要方法:

  • 从现有的文献中收集了470种复制启动蛋白 (RIP).
  • 确定了35个与RIP相关的保护域.
  • 开发了一个评分系统,以评估基因间序列的可能性作为潜在的oriVs,整合RIP信息和保存特征.

主要成果:

  • OriV-Finder可以准确地识别同类的RIP基因.
  • 服务器使用基于优先级的评分系统来评估基因间序列成为潜在的oriVs的可能性.
  • 为oriVs,RIPs和基因组集成的可视化模块促进了分析和验证.

结论:

  • OriV-Finder提供了一种有效和准确的方法来识别细菌等离子体复制起源.
  • 网络服务器简化了oriVs的分析和验证,帮助研究等离子体生物学.
  • OriV-Finder是免费访问的,促进科学界更广泛的使用.