68个Ga标记单域抗体的免疫PET成像,用于检测瘤TIGIT表达
Dinghu Weng1,2,3,4, Rong Guo5,6, Yu Gao5,6
1Shanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and Health Sciences, 201318 Shanghai, China.
Molecular pharmaceutics
|April 29, 2025
概括
研究人员开发了一种新型的正电子发射断层扫描 (PET) 放射追踪器,Ga-NOTA-NABT3A1,以可视化T细胞免疫球蛋白和免疫受体氨酸基基抑制动机域 (TIGIT) 表达. 这种标志物显示出在瘤中非侵入性评估TIGIT的潜力,以提供免疫治疗指导.
科学领域:
- 免疫学 免疫学 免疫学
- 放射化学 放射化学是指辐射化学.
- 分子成像学分子成像学
背景情况:
- 瘤微环境中的T细胞免疫球蛋白和免疫受体氨酸基抑制动机域 (TIGIT) 表达与抗TIGIT免疫疗法的疗效相关.
- 需要使用非侵入性方法来评估TIGIT表达,以指导治疗决策.
研究的目的:
- 为了开发和表征一种基于TIGIT准单域抗体 (sdAb) 的正子发射断层扫描 (PET) 放射追踪器,Ga-NOTA-NABT3A1.1.
- 评估开发的放射性追踪剂的体外稳定性,特异性和体内性能.
主要方法:
- 修改NABT3A1 sdAb的NOTA衍生品,用于68Ga放射性标记.
- 在PBS和FBS中评估放射性追踪剂的稳定性和使用TIGIT表达黑色素瘤细胞 (A375-TIGIT) 的特异性.
- 在A375-TIGIT瘤携带的裸体小鼠体内免疫PET成像,随后进行生物分布和免疫组织化学分析.
主要成果:
- [68Ga]Ga-NOTA-NABT3A1已成功合成,具有高放射性化学产量 (70.56 ± 1.14%) 和纯度 (>95%).
- 放射追踪仪在体外表现出良好的稳定性,在体内A375-TIGIT瘤中表现出良好的特异性积累.
- 在注射后0.5小时观察到3.86±0.29%注射剂量/g的最大瘤吸收率,由生物分布研究证实.
结论:
- 由NABT3A1衍生的PET射线追踪器Ga-NOTA-NABT3A1成功开发.
- 这种新型的追踪器在对瘤TIGIT表达的非侵入性评估中表现出有希望的特征.
- 这些发现表明,这对指导针对TIGIT的免疫疗法具有潜在的临床有用性.
相关概念视频
Positron Emission Tomography
3.9K
Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
3.9K
Imaging Studies II: Positron Emission Tomography and Scintigraphy
39
Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET
Fundamental Principles of PET
39
Radiological Investigation III: Pulmonary Angiogram and PET Scan
73
Radiological investigations are paramount in the diagnosis and management of various pulmonary diseases. Two essential investigations are the Pulmonary Angiogram and the Positron Emission Tomography (PET) Scan.
Pulmonary Angiogram
A Pulmonary Angiogram is an invasive procedure involving injecting a contrast medium through a catheter threaded into the pulmonary artery or the right side of the heart to visualize the pulmonary vasculature. Computed Tomography (CT) scans have mainly replaced this...
Pulmonary Angiogram
A Pulmonary Angiogram is an invasive procedure involving injecting a contrast medium through a catheter threaded into the pulmonary artery or the right side of the heart to visualize the pulmonary vasculature. Computed Tomography (CT) scans have mainly replaced this...
73
Immunogold Electron Microscopy
3.9K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
3.9K


