一种与视网膜母细胞瘤相关的蛋白质通过SHR/SCR网络促进Populus中的偶然根部发育和二次壁形成
Haoran Qi1,2, Luyang Shan1, Yaoyao Zhu1
1State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, China.
视网母细胞瘤相关 (RBR) 蛋白调节植物的发育. 在中过度表达PeRBR,通过调高细胞循环和素生物合成基因,增强了偶然的根和二次体生长.
科学领域:
- 植物分子生物学 植物分子生物学
- 木植物开发公司
- 细胞循环规则 细胞循环规则
背景情况:
- 视网母细胞瘤相关 (RBR) 蛋白质是细胞周期,分化和应激反应的保守调节者.
- SHR/SCR网络控制植物中的偶然根 (AR) 形态发生和二次壁的形成.
研究的目的:
- 在混合树 (Populus deltoides × P. euramericana) 中功能性地表征PeRBR.
- 调查PeRBR与SHR/SCR网络在AR和二次墙壁开发中的监管相互作用.
主要方法:
- 转基因树产生过度表达PeRBR.
- 双分子光补充 (BiFC) 和定量实时PCR (qRT-PCR) 用于相互作用和基因表达分析.
- 转录基因剖析用于识别差异表达基因 (DEGs).
主要成果:
- 过度表达PeRBR显著增强了AR系统架构和二次体发育,增加了体层和素沉积.
- PeRBR与PeSCR直接相互作用,并通过转录上调关键基因,包括PeSHR,PeCYCD6;1和PeWOX5.5.
- 转录基因分析显示,烯胺生物合成途径的丰富,在OE_PeRBR系中显著上调宁和纤维素合成基因.
结论:
- 在SHR/SCR网络中,PeRBR充当中央监管节点.
- 在木质物种中,PeRBR通过转录调节细胞周期和细胞壁生物合成基因来协调AR发育和二次壁的形成.
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