铁通过重新编程碳代谢来调节依赖酸盐的甲性细菌的生长和活动
Xiangwu Yao1,2, Meng Zhang1,2,3, Mike S M Jetten4
1State Key Laboratory of Soil Pollution Control and Safety, Zhejiang University, Hangzhou 310058, China.
Environmental science & technology
|April 29, 2025
概括
在微生物中储存铁的代谢成本很高. 这项研究揭示了铁作为细菌中的代谢开关,重新编程路径以平衡生长和活动,以增强功能.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 环境科学 环境科学
背景情况:
- 微生物需要铁来实现基本功能,但过量的铁可能是有毒的.
- 费里丁和其他基于蛋白质的部分控制铁的稳态.
- 储存铁的高能源成本引发了关于微生物代谢调节的问题.
研究的目的:
- 研究铁的储存如何影响微生物的新陈代谢和活动.
- 确定细菌中由铁调节的新型代谢重编程途径.
- 了解微生物生长和在不同铁含量下代谢功能之间的平衡.
主要方法:
- 利用依赖酸盐的甲类细菌作为模型系统.
- 采用同位素标记和meta-omics分析来追踪代谢转变.
- 应用显微镜和能量分散光谱学可视化铁储存和细胞变化.
主要成果:
- 观察到细菌丰富度和依赖甲的脱活性之间的断开.
- 在暴露于高铁 (40μM Fe2+) 的细胞中鉴定出富含铁的纳米粒子.
- 检测到铁代谢和甲氧化与脱结合的基因表达增加.
- 记录了从卡尔文循环到碳固定的氨酸途径的代谢转移.
结论:
- 铁作为一种代谢开关,重新编程路径以协调物质和能量代谢.
- 代谢调整提高了甲性细菌同时去除和碳的能力.
- 这些发现为环境应用操纵微生物代谢提供了洞察力.
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