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在动脉样硬化中使用绿茶加压热水提取物:关于细胞,动物和分子机制的多方法研究

Rahni Hossain^1,2,3, Anawat Kongchain², Moragot Chatatikun^2,3

¹College of Graduate Studies, Walailak University, Nakhon Si Thammarat 80160, Thailand.

Antioxidants (Basel, Switzerland)

|April 29, 2025

概括

绿茶提取物通过减少氧化应激和改善脂质样本来对抗动脉样硬化,显示出作为天然心血管风险降低剂的潜力. 它的化合物有效地保护细胞,并提供与他类药物相比的好处.

科学领域:

心血管研究研究心血管研究
药理学药理学是指药理学的学科.
自然产品化学自然产品化学

背景情况:

动脉样硬化是一种由氧化应激和脂质失调驱动的慢性炎症性疾病,是心血管疾病的主要原因.
绿茶加压热水提取物 (GPHWE) 含有丰富的抗氧化剂,如儿茶素和黄类.
对于动脉样硬化的现有治疗方法有局限性,这推动了寻找新的治疗策略的研究.

研究的目的:

研究GPHWE在预防动脉样硬化和减轻心血管风险方面的潜力.
阐明GPHWE对抗氧化应激和脂质失衡的保护作用的机制.
评估GPHWE在体外和体内疗效,将其与标准药物进行比较.

主要方法:

在体外抗氧化剂测试 (DPPH,ABTS,氧,氧化清理).
在体外评估GPHWE对巨细胞对氧化LDL (Ox-LDL) 的保护作用.
在体内研究使用高脂肪饮食 (HFD) 鼠标模型来评估脂质配置文件和氧化应激标志物.
分子对接模拟用于预测GPHWE组件与关键动脉样硬化点之间的相互作用.
在 UHPLC-QTOF-MS 用于 GPHWE 中提前识别甲基素.

主要成果:

在实验室中,GPHWE显示出显著的抗氧化活性.
GPHWE保护巨细胞免受Ox-LDL诱导的损伤,减少细胞毒性和亡.
在HFD小鼠中,GPHWE改善了脂质特征 (降低了总胆固醇,LDL,甘油三) 和减少了氧化应激 (降低了MDA水平),效果与Simvastatin相似.
分子对接揭示了 катехин与LOX-1,HMG-CoA减少酶和Nrf2.2等点的强烈结合.
暂时确定了关键儿茶素 (EGCG,ECG,EGC).

结论:

GPHWE具有强大的抗氧化和抗动脉样硬化特性.
这些机制包括抗氧化作用,脂质调节和斑块稳定.
作为一种用于预防动脉样硬化和降低心血管风险的天然治疗剂,GPHWE显示出显著的潜力.

关键词:

卡梅利亚·辛内西斯 (Camellia sinensis) 是一种植物.灭症 (apoptosis) 是一种死亡的过程.动脉样硬化动脉样硬化绿茶绿茶是一种绿茶.氧化LDL的LDL已经氧化了.加压热水提取物 (GPHWE) 的使用

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