卢比康是氧化应激中介的DNA损伤的关键分子,在卵巢粒状细胞中
Kiyotaka Yamada1, Masami Ito1, Haruka Nunomura1
1Department of Obstetrics and Gynecology, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
Antioxidants (Basel, Switzerland)
|April 29, 2025
概括
衰老通过氧化应激 (OS) 损害了卵巢功能. 这项研究发现,三糖通过激活自和抑制Rubicon - - 颗粒状细胞中DNA损伤的关键媒介 - - 来增强细胞对OS的抵抗力.
科学领域:
- 生殖生物学 生殖生物学
- 细胞衰老 细胞衰老
- 老龄化的分子机制.
背景情况:
- 衰老与卵巢氧化压力 (OS) 的增加有关,这会对女性生育能力产生负面影响.
- 粒状细胞 (GCs) 对于卵泡发育至关重要,容易受到OS诱导的损伤.
- 在GC中,OS和自之间的相互作用需要进一步阐明.
研究的目的:
- 为了研究氧化应激和粒粉细胞中自的关系.
- 为了确定可以增强细胞抵抗氧化应激的化合物.
- 在OS下探索Rubicon作为GCs中DNA损伤的调解者的作用.
主要方法:
- 利用人类GC细胞系 (HGrC1) 来研究OS效应.
- 研究过氧化 (H2O2) 对细胞活力和自活动的影响.
- 评估了自活化激活剂三糖对H2O2诱导的细胞毒性的影响.
- 分析了Rubicon表达,DNA损伤标记和反应性氧物种的产生.
主要成果:
- 过氧化通过DNA损伤损害了HGrC1细胞的活力.
- 自激活在HGrC1细胞中赋予了OS耐药性.
- 一种分糖酸特雷哈洛斯 (Trehalose) 作为一种自活化剂,增强了OS的抗性,减少了Rubicon的表达.
- 鲁比康的淘汰减轻了OS诱导的DNA损伤,而其过度表达加剧了损伤并降低了细胞活力.
结论:
- 特雷哈洛斯通过双向激活自和抑制鲁比康来增强GC中的OS抵抗力.
- 在OS下,Rubicon被确定为GC中DNA损伤的关键调解者.
- 糖具有改善卵巢功能在衰老和不孕症以及其他与OS相关的疾病中的潜力.
相关概念视频
DNA Damage can Stall the Cell Cycle
9.0K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.0K
DNA Damage Can Stall the Cell Cycle
2.5K
2.5K
Nucleotide Excision Repair
3.4K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
3.4K
The DNA Replication Fork
35.3K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
35.3K
Peroxisomes
8.4K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
8.4K
Crossing Over
4.0K
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
4.0K


