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由G-CSF诱导的紧急颗粒形成调节了小鼠中性粒细胞效应器的功能

Jonah K Stephan¹, Taylor Knerr¹, Collin K Wells¹

¹Center for Cardiometabolic Science, Christina Lee Brown Envirome Institute, University of Louisville School of Medicine, 580 South Preston Street - Rm 204B, Louisville, KY, USA.

Stem cell reviews and reports

|April 29, 2025

概括

由G-CSF触发的紧急颗粒形成,产生具有功能改变的中性粒细胞. 这些中性粒细胞表现出受损的活性氧物种生产和NETosis,但增强了弹性酶分泌和细胞因子表达,影响了炎症.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

中性粒细胞是关键的免疫反应者,它们的功能是在颗粒形成过程中被编程的.
在严重炎症期间激活紧急颗粒形成,以满足中性粒细胞需求的增加.
紧急颗粒形成对中性粒细胞的功能后果尚不清楚.

研究的目的:

调查紧急颗粒形成对中性粒细胞的功能影响.
评估通过紧急颗粒形成产生的中性粒细胞如何影响体内炎症反应.

主要方法:

用G-CSF治疗小鼠,以诱导紧急颗粒形成.
评估了中性粒细胞功能,包括ROS产生,NETosis,弹性酶分泌和细胞因子表达.
用于评估G-CSF治疗小鼠的炎症反应,使用了因齐莫桑诱导的腹膜炎的体内模型.

主要成果:

来自G-CSF治疗小鼠的中性粒细胞表现出ROS产量受损和NETosis.
这些中性粒细胞表现出中性粒细胞弹性酶分泌量增加和促炎性细胞因子 (TNF-α,IL-1β,IL-1α,IL-12α,CCL2) 的表达升高.
在体内,接受G-CSF治疗的小鼠接受了齐莫桑的挑战,表现出腹中性粒细胞CCL2表达的增加和巨细胞积累的增强.

结论:

紧急颗粒形成重编程中性粒细胞,导致增强的免疫调节功能.
这些中性粒细胞在局部组织炎症期间协调随后的巨细胞反应中发挥着关键作用.

关键词:

细胞因子细胞因子在G-CSF中使用.颗粒构造是一种颗粒构造.血液形成血液形成血液形成炎症炎症是一种炎症.作为一个中性粒细胞.

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