便携式SERS用于基于唾液检测口腔前恶性病变和癌症:迈向临床实施的一步
Arvind Kaushik1, Sneha Senapati2, Neha Agrawal3
1Department of Physics, Indian Institute of Technology Delhi, New Delhi 110016, India.
概括
表面增强拉曼光谱 (SERS) 能够在早期阶段有效检测口腔癌. 这种方法分析了前恶性和恶性样本的光谱概况,提供了高诊断准确度,改善了患者的治疗结果.
科学领域:
- 生物医学光谱学 生物医学光谱学
- 化学分析 化学分析
- 癌症的诊断 癌症的诊断
背景情况:
- 全球口腔癌的发病率正在上升,主要是由于生活方式因素和检测延迟.
- 早期诊断对于有效治疗和改善口腔癌生存率至关重要.
- 目前的诊断方法在检测早期或前恶性口腔病变时可能受到限制.
研究的目的:
- 评估表面增强拉曼光谱 (SERS) 的诊断准确性,以检测前恶性和恶性阶段的口腔癌.
- 在与口腔病变不同阶段相关的SERS概况中识别不同的光谱生物标志物.
- 评估SERS与多变量分析结合用于早期口腔癌检测的潜力.
主要方法:
- 分析了99个临床样本 (39个恶性,28个前恶性,32个健康对照) 使用无标签的银纳米基质SERS基质.
- 描述SERS光谱以确定与无机代谢物和蛋白质相关的峰值.
- 应用多变量分析和比度分析 (2130厘米-1 / 1435厘米-1) 来区分样本组.
主要成果:
- SERS光谱显示了恶性和前恶性样本的明显峰值,与代谢物和蛋白质度变化有关.
- 恶性样本的特征是提升的硫酸酸盐水平,而抗氧化剂 (尿酸,桑,低桑) 是前恶性样本的关键.
- 比例度分析显示前恶性病变在2-3个和恶性样本>3之间,在多变量分析中93.5%的灵敏度和92.7%的特异性.
结论:
- 与多变量分析相结合的SERS分析显示了口腔癌检测的高诊断准确性.
- 该研究确定了特定的光谱生物标志物,表明了前恶性和恶性口腔疾病.
- 塞尔斯为口腔癌的早期检测提供了一个有希望的,非侵入性的工具,可能有助于临床决策和拯救生命.
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