通过病原体效应器ApcE12对混合竹BDDnaJ的向调节了未折叠的蛋白质反应
Fengying Luo1, Xinmei Fang2, Haiyan Luo1
1College of Forestry, Sichuan Agricultural University, Chengdu, China.
Molecular plant pathology
|April 29, 2025
概括
这项研究揭示了真菌效应剂ApcE12如何操纵竹子的展开蛋白质反应 (UPR) 和编程细胞死亡 (PCD) 途径. 了解这种相互作用是开发竹子疾病耐药性的新策略的关键.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 生物化学 生物化学
背景情况:
- 由 *Arthrinium phaeospermum* 引起的射病威胁着竹子 (*Bambusa pervariabilis* × *Dendrocalamopsis grandis*) 的价值.
- 真菌病原体经常分泌效应物来抑制宿主免疫力,以成功感染.
研究的目的:
- 调查真菌效应剂ApcE12在调节竹植物免疫力中的作用.
- 阐明ApcE12与宿主蛋白BDClp和BDDnaJ之间的相互作用.
- 了解ApcE12如何操纵展开的蛋白质反应 (UPR) 和编程细胞死亡 (PCD) 途径.
主要方法:
- 酵母两杂交测定以确认蛋白质与蛋白质的相互作用.
- 双分子光补充 (BiFC) 用于体内相互作用验证.
- 为了评估直接约束性,GST拉下测试.
- 基因沉默技术用于评估宿主蛋白的功能作用.
- 对UPR信号和PCD激活的分析.
主要成果:
- ApcE12与宿主蛋白BDDnaJ直接相互作用,BDDnaJ是UPR.12的一个调节器.
- 沉默BDDnaJ通过减少UPR,激活PCD和阻止感染来增强植物的耐药性.
- BDDnaJ 调节了 BDBiP 蛋白质的稳定性.
- ApcE12操纵BDDnaJ以增强UPR信号和抑制PCD,促进真菌感染.
结论:
- 效应物可以利用宿主的UPR和PCD通路来克服植物的防御.
- ApcE12和BDDnaJ之间的相互作用是病原体毒性的一个关键机制.
- 研究结果为开发新型抗药策略提供了洞察力,以对抗竹病.
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