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在米索和大豆源酵母Zygosaccharomyces sp sp的CRISPR-Cas9基因组编辑中

Tomoo Ogata¹, Kotori Koide¹, Shiori Kudou¹

¹Department of Life Engineering, Maebashi Institute of Technology.

The Journal of general and applied microbiology

|April 29, 2025

概括

使用CRISPR-Cas9的基因组编辑成功修改了Zygosaccharomyces酵母菌株,为米索和大豆生产提供了新的育种技术. 这种进步允许有针对性的基因突变和改善酵母特征.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

这两种植物是Zygosaccharomyces rouxii和Zygosaccharomyces sp. 是在米索和大豆生产中至关重要的酵母.
开发这些酵母的先进育种技术对于优化发酵过程至关重要.

研究的目的:

在Zygosaccharomyces酵母菌株中建立和应用CRISPR-Cas9基因组编辑.
为了研究基因组修饰的效率和结果,无论是 haploid 和 allodiploid 菌株.

主要方法:

克里斯普尔-卡斯9系统组件 (卡斯9基因和导向RNA) 来自单个等离子体.
向基因修饰ZygoLEU2基因在单体Z. rouxii和全二体Zygosaccharomyces sp.中的向基因修饰. 菌株. 菌株. 这些菌株.
应用CRISPR-Cas9来突变HOG1基因,影响盐和度适应.

主要成果:

在ZygoLEU2基因中成功诱导单核酸插入和删除,从而导致白素辅性转化.
在全二倍体菌株中进行基因组编辑导致了两个亚基因组的突变,在某些情况下,染色体的删除.
在高盐和高度条件下,HOG1基因的突变显著损害了酵母的生长.

结论:

克里斯普尔-Cas9是一种有效的工具,用于基因组修改Zygosaccharomyces酵母.
这项技术促进了针对性基因操纵,以改善食品生产中的酵母菌株.
了解基因功能,如压力反应中的HOG1,通过基因组编辑来增强.

关键词:

克里斯普尔-Cas9是什么意思这是一种Zygosaccharomyces酵母酵母.基因组编辑基因组编辑

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