采用气体利用甲基生物的自动化高性能封闭批量栽培方法
Walter Hofmann1,2, Marco Orthofer3, Nicolás Salas Wallach1,2
1Archaea Physiology & Biotechnology Group, Department of Functional and Evolutionary Ecology, Universität Wien, Djerassiplatz 1, 1030, Wien, Austria.
AMB Express
|April 29, 2025
概括
一种新的气体和压力控制器 (GPC) 装置使得在封闭的微生物培养中能够自动控制头部气体压力. 这项技术显著提高了古生物中的甲生产率,克服了气体发酵的先前限制.
科学领域:
- 微生物生物技术 微生物生物技术
- 生物工艺工程是生物工艺工程.
- 合成生物学 合成生物学
背景情况:
- 推进气体发酵的微生物细胞工厂需要了解它们的生理和生物技术限制.
- 目前的方法经常在控制头部气体压力方面遇到局限性,阻碍了微生物的最佳生长和产品形成.
- 自动化控制系统对于封闭式种植系统的精确调节至关重要.
研究的目的:
- 建造和运行一个新的气体和压力控制器 (GPC),用于自动控制封闭培养瓶中的头部气体压力.
- 在精确控制的气体条件下,研究自营养,营养和甲产物古生物的生理和生物技术特征.
- 通过消除生长和甲形成期间的气体限制来量化新的生理极限.
主要方法:
- 开发和实施气体和压力控制器 (GPC) 用于实时自动加气,散热,监测和调节头部空间体积.
- 使用GPC培育了四种自性,性甲原体古生物 (Methanothermobacter marburgensis,Methanotorris igneus,Methanocaldococcus jannaschii,Methanocaldococcus villosus) 的研究结果.
- 测试GPC与Methanococcus maripaludis的功能,以长期进行自主栽培.
主要成果:
- 对于测试的古生物,确定了前所未有的高最大特定甲生产率 (qCH4) 值,包括M. marburgensis (169.59 ± 12.52 mmol g−1 h−1) 和M. igneus (420.21 ± 89.46 mmol g−1 h−1).
- 马尔堡氏菌的qCH4比传统的封闭批次培养高10倍以上,与养批次系统相比.
- GPC证明了对M. maripaludis的可靠,安全和自主长期功能.
结论:
- 新型GPC装置能够实现最佳的头部空间压力控制,为各种气体发酵生物技术过程提供灵活性.
- 该设备在半连续封闭批次模式下促进了近乎最佳的种植条件,有助于分析限制因素.
- GPC支持自动化生物质生产的自营性,性甲基生物.
相关概念视频
01:26Microbes and Methanogenesis
Methanogenesis is a critical microbial process in anaerobic ecosystems responsible for the biological production of methane, a potent greenhouse gas and valuable biofuel. This metabolic pathway is primarily facilitated by methanogenic archaea, which thrive in anoxic environments such as wetlands, sediments, and animal gastrointestinal tracts. The absence of oxygen in these habitats prevents aerobic respiration, thereby favoring alternative biochemical pathways for organic matter degradation.In...
01:29Bioreactor Design and Operational System
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
01:18Bioreactor Controls-II
In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
01:14Batch vs Continuous Culture
Fermentation is a foundational biotechnological process used to produce pharmaceuticals, biofuels, enzymes, and food additives. Among industrial strategies, batch and continuous fermentation are the two most widely applied. Although both rely on microbial conversion of substrates into desired products, they differ markedly in operation, productivity, and suitability for specific applications.Batch fermentation occurs in a closed system in which nutrient media and inoculum are added at the...
01:23Fed-Batch Culture
Fed-batch culture is a widely used bioprocessing strategy combining aspects of batch culture with controlled substrate feeding to optimize cell growth and product formation. In this semi-closed system, nutrients are strategically added during fermentation, while the accumulated products and biomass remain within the bioreactor until the end of the operation. This controlled addition of substrates allows for better management of growth kinetics, nutrient limitation, and metabolite...
01:14Scale-Up Processes
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...