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一个SNRK2-HAK调节模块赋予了玉米盐分耐受性的自然变化

Ming Zhang^1,2,3, Xueyan Zhou⁴, Limin Wang⁴

¹State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing, China. zhangming@henu.edu.cn.

Nature communications

|April 29, 2025

概括

玉米盐的耐受性涉及射击的排除. 两个激酶,ZmSnRK2.9和ZmSnRK2.10,激活ZmHAK4,增强运输和改善作物盐耐受性.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
农作物科学农作物科学

背景情况:

射击排除对于作物盐耐受性至关重要.
在这个过程中,高亲和度K+传送器 (HAK) 传送器家族是关键.
这些载体的转录后调节在很大程度上仍未被探索.

研究的目的:

研究SnRK2激酶在玉米中所扮演的角色.
阐明ZmHAK4.4的转录后调节机制.
确定培育耐盐玉米品种的潜在目标.

主要方法:

酵母两杂交测试以确定蛋白质相互作用.
在体外激酶测试以确定激酶活性.
定量PCR分析基因表达水平.
位点定向突变发生,以研究酸化位点.

主要成果:

ZmSnRK2.9和ZmSnRK2.10激活ZmHAK4的运输活动.
ZmSnRK2.9/ZmSnRK2.10的激酶活性是由盐应激诱导的.
在Ser5中通过ZmSnRK2.9/ZmSnRK2.10对ZmHAK4的酸化增强了的排斥.
在ZmSnRK2.10中的自然促进体删除降低了其转录水平,增加了射击含量.

结论:

ZmSnRK2.9和ZmSnRK2.10是ZmHAK4活性和玉米盐耐受性的关键调节者.
通过SnRK2激酶对ZmHAK4的转录后激活是盐耐受性的重要机制.
有利的ZmHAK4和ZmSnRK2.10等位基因可用于培育耐盐玉米.

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