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基于DNA酶的色度生物传感器用于快速检测Shigella flexneri

Manikandan Murugan¹, Elangovan Saranya¹, Mohandass Ramya²

¹Molecular Genetics Laboratory, Department of Genetic Engineering, SRM Institute of Science and Technology, College of Engineering and Technology, Kattankulathur, Chengalpattu, Chennai, Tamil Nadu, 603 203, India.

Scientific reports

|April 29, 2025

概括

一种新的色度测定方法可以快速检测出Shigella flexneri,这是一种危险的细菌,会导致石病. 这种使用aptamer和碳纳米管的基于DNA酶的方法为食品和水安全提供了一个用户友好的工具.

科学领域:

微生物学微生物学
纳米技术纳米技术
生物技术是生物技术.

背景情况:

石格拉柔性菌 (Shigella flexneri) 引起石格洛症,一种严重的腹疾病.
目前的检测方法缓慢且不方便.
快速诊断对于食品和水安全至关重要.

研究的目的:

开发一种快速,准确的S. flexneri.诊断测定方法.
使用阿普坦和DNA酶技术进行检测.

主要方法:

修改的S. flexneri特定的HGD-aptamer和单壁碳纳米管 (SWCNTs).
开发了一种利用DNA酶活动的色度检测试验.
验证了测定灵敏度,特异性,并与PCR进行比较.

主要成果:

对于S. flexneri. 的检测极限达到51 CFU/mL.
证明了选择性检测,没有其他细菌的信号干扰.
测试性能与PCR方法相比.

结论:

开发的测定方法是S. flexneri检测的快速,经济高效和用户友好的工具.
增强食品安全,环境监测和公共卫生监测的潜力.
对于病原体识别的创新方法.

关键词:

斯·弗莱克斯内里 (S. Flexneri) 是一个柔软的动物.测色系统的颜色测量系统.这就是DNAzyme DNA酶.在 HGD 吸收剂中,HGD 吸收剂是单壁碳纳米管是一种单壁碳纳米管.

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