与类风湿性关节炎相关的间歇性肺病的DNA甲基组生物标志物反映了肺纤维化途径,这是一项探索性病例控制研究

相关实验视频

Updated: May 9, 2025

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

Bartosz Kaczmarczyk¹, Carlos de la Calle-Fabregat², Adrian Conde¹

¹Experimental and Observational Rheumatology and Rheumatology Unit, Instituto Investigacion Sanitaria-Hospital Clinico Universitario de Santiago, Santiago de Compostela, Spain.

Scientific reports

|April 29, 2025

概括

DNA甲基化分析揭示了与类风湿性关节炎相关的间歇性肺部疾病 (RA-ILD) 的明显模式. 这些发现表明,潜在的血液生物标志物可用于早期检测和了解RA-ILD患者的肺纤维化.

科学领域:

基因组学就是基因组学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
肺部病理学肺部病理学

背景情况:

风湿性关节炎相关的间歇性肺病 (RA-ILD) 严重影响患者的生活质量和生存率.
目前对RA-ILD的理解和临床管理需要显著改进.
在此之前,DNA甲基化分析尚未应用于RA-ILD患者样本.

研究的目的:

为了研究RA-ILD患者的DNA甲基化模式.
为了确定RA-ILD和相关的肺纤维化潜在的血液生物标志物.
在RA-ILD中探索DNA甲基化和纤维化通路之间的关系.

主要方法:

对32名RA-ILD患者和32名没有ILD的RA患者的血液样本进行了比较DNA甲基化分析.
使用统计方法来确定差异甲基化位置 (DMP) 和区域 (DMR).
进行了基因组丰富分析和转录因子结合部位分析.

主要成果:

在RA-ILD患者中确定了6,679个差异甲基化位置 (DMP) 和576个差异甲基化区域 (DMR).
丰富的基因组与纤维化,单细胞和膜巨细胞基因表达,线粒体和Rho GTPases有关.
观察到肝脏X受体元素 (LXRE) 附近的转录因子结合部位的独特丰富,这是已知的组织纤维化因素.

结论:

在RA-ILD患者中存在不同的DNA甲基化模式.
这些甲基化变化与关键的纤维化通路有关,特别是在肺组织中.
DNA甲基化分析显示,开发RA-ILD及其纤维化并发症的血液生物标志物具有前途.

关键词:

生物标志物生物标志物通过DNA甲基化.表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.间歇性肺病间歇性肺病肺部纤维化肺部纤维化类风湿性关节炎类风湿性关节炎类风湿性关节炎

更多相关视频

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

Imaging Features of Systemic Sclerosis-Associated Interstitial Lung Disease

Imaging Features of Systemic Sclerosis-Associated Interstitial Lung Disease

Published on: June 16, 2020

相关概念视频

Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...