益生菌Pediococcus acidilactici 72 N的工业生产和功能分析,以作为猪料添加剂的潜在用途
Nay Zin Myo1,2, Ratchnida Kamwa1,2, Benjamas Khurajog1
1Department of Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Scientific reports
|April 29, 2025
概括
在工业规模生产Pediococcus acidilactici 72N (P72N) 益生菌时,使用了成本效益低的食品级介质. 这种优化过程显著提高了可活细胞的数量,并降低了养猪应用的成本.
科学领域:
- 微生物学和生物技术
- 动物科学动物科学
- 食品科学 食品科学 食品科学
背景情况:
- 开发具有成本效益的益生菌生产方法对于畜牧业至关重要.
- 泰国本土的来自猪的Pediococcus acidilactici 72N (P72N) 是一个有前途的益生菌候选人.
- 优化生长基对于最大限度地提高益生菌产量和功能性质至关重要.
研究的目的:
- 开发和优化一种食品级,经济高效的媒介,用于工业规模生产Pediococcus acidilactici 72N (P72N).
- 评估在优化介质中培养的P72N的功能特征和代谢特征.
- 评估优化的介质是否适合大规模P72N发酵作为猪料添加剂.
主要方法:
- 使用常规和统计优化技术对食品级碳和源进行选.
- 在受控条件下的5L和50L发酵器中进行规模化发酵试验 (37°C,pH6.5,每分钟120转).
- 评估可活细胞数量,对环境压力的耐受性和代谢概况 (包括1,4-DHNA和ILA).
主要成果:
- 优化的介质 (德克斯特,酵母提取物,酸,酸,二酸,Tween 80,硫酸,硫酸) 与商业MRS介质相比,显著增加了可活细胞数量.
- 在12小时内实现了工业规模的发酵>9.60 log CFU/mL,降低了67%-86%的生产成本.
- P72N表现出增强的应激耐受性,并产生更高水平的生物活性代谢物,包括1,4-二基-2-纳酸 (1,4-DHNA) 和乳酸 (ILA).
结论:
- 开发的食品级介质对于工业规模生产Pediococcus acidilactici 72N (P72N) 是非常有效和具有成本效益的.
- 优化的发酵过程支持P72N.的高产量和增强的功能性质.
- 通过这种方法生产的P72N是养猪养殖中作为益生菌料添加剂的可行候选者.
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