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PYGO2调节IL10并通过ESCC进展发挥免疫抑制作用

Fereshteh Ahmadian Shalchi^1,2, Nayyerehalsadat Hosseini³, Fatemeh Nourmohammadi⁴

¹Department of Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

BMC molecular and cell biology

|April 29, 2025

概括

在食道状细胞癌 (ESCC) 中,PYGO2和IL10mRNA过度表达,与瘤进展相关. PYGO2可能调节IL10,这表明它在ESCC发育中的作用.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
胃肠病学胃肠病学

背景情况:

食道状细胞癌 (ESCC) 是一种具有高死亡率的侵袭性癌症.
该Wnt通路对于细胞增殖和分化至关重要.
PYGO2和IL10是Wnt通路中的关键参与者.

研究的目的:

研究ESCC中PYGO2和IL10表达之间的相关性.
分析基因表达和临床病理特征之间的关系.
探索ESCC中PYGO2和IL10之间的功能相互作用.

主要方法:

实时定量PCR (RT-qPCR) 用于评估ESCC组织和细胞系中的PYGO2和IL10mRNA水平.
在ESCC细胞系 (KYSE-30,YM1) 中诱导PYGO2表达,以观察对IL10的影响.
统计分析以将基因表达与瘤分化和入侵深度相关联.

主要成果:

在ESCC中观察到PYGO2 (31.0%) 和IL10 (51.7%) mRNA的显著过度表达.
过度表达PYGO2和IL10显示出显著的正相关性 (p=0.007).
同时的过度表达与较高的瘤等级和入侵深度相关 (p<0.01,p<0.05).
诱导PYGO2表达改变了ESCC细胞中的IL10水平.

结论:

PYGO2可能通过Wnt/β-catenin通路调节IL10,这表明PYGO2/IL10轴在ESCC中的潜在致癌作用.
PYGO2和IL10之间的相互作用可能会促进ESCC的入侵和恶性瘤.
针对PYGO2/IL10轴可以为ESCC提供新的治疗策略.

关键词:

IL10 IL10 已经开始了这就是PYGO2的原因.共同的过度表达.定量实时PCR可以使用.在Wnt/β-catenin路径中.

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