通过刺激拉曼散射显微镜对药物材料中的固态复杂性的描述
Elina A Harju1, Teemu Tomberg1,2, Lea Wurr1
1Drug Research Program, Division of Pharmaceutical Chemistry and Technology, University of Helsinki, Helsinki 00014, Finland.
Analytical chemistry
|April 30, 2025
概括
刺激拉曼散射 (SRS) 显微镜现在可以特征复杂的固态混合物,包括多种形式的乳糖,具有高空间分辨率. 这种先进的无标签技术为制药应用提供了敏感检测和定量分析.
科学领域:
- 材料科学 材料科学 材料科学
- 分析化学 分析化学
- 制药科学 制药科学
背景情况:
- 描述复杂的固态混合物,特别是具有同一化合物的多重多态形式的混合物,会带来重大分析挑战.
- 既定的方法往往缺乏空间分辨率或灵敏度,无法在单个样本中完全解析和量化这些多样化的形式.
研究的目的:
- 引入和验证一种使用刺激拉曼散射 (SRS) 和总频率生成 (SFG) 进行复杂固态混合物的表征的新型多模式显微镜方法.
- 证明该技术在分解和量化乳糖的各种固态形式,包括晶体和无形相中的能力.
主要方法:
- 使用刺激拉曼散射 (SRS) 显微镜与总频率生成 (SFG) 显微镜相结合.
- 采用无标签多式显微镜方法进行成像和分析.
- 应用该技术来表征五种晶体和一种无形形式的乳糖,以及商用药品等级.
主要成果:
- 成功描述和解决六种不同的固态形式的乳糖,包括两种最近定义的无水形式.
- 可视化了这些固态形式的空间分布,具有亚微米分辨率,可以检测痕迹水平.
- 提供了各种乳糖产品中固态成分的定量估计.
结论:
- 用SFG增强的SRS显微镜,提供复杂混合物的敏感,特定和空间分辨的固态特征.
- 这种先进的技术超越了详细的固态分析的已建立的非空间解析方法的能力.
- 这些发现对制药品质量控制和材料科学研究有重大影响.
相关概念视频
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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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