相关实验视频

Updated: May 9, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

揭露ADAR和APOBEC蛋白质基因家族的RNA编辑

Alexander Modestov^1,2, Anton Buzdin^1,2,3,4, Maria Suntsova¹

¹Institute of Personalized Oncology, I.M. Sechenov First Moscow State Medical University, 119991 Moscow, Russia.

Frontiers in bioscience (Landmark edition)

|April 30, 2025

概括

通过ADAR和APOBEC酶调解的RNA编辑会影响基因表达和蛋白质结构. 这篇评论探讨了RNA编辑作为癌症进展的生物标志物,分析编辑水平和特定的基因.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
生物化学生物化学

背景情况:

RNA编辑是一个关键的转录后过程,它修改RNA分子.
像ADAR (作用于RNA的腺氨酸脱氨酶) 和APOBEC (类似于催化聚的apolipoprotein B mRNA编辑) 这样的酶是RNA编辑的核心.
异常的RNA编辑与瘤的发展和进展有关.

研究的目的:

审查RNA编辑参数作为癌症进展的生物标志物的潜力.
突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出突出
讨论加速RNA编辑事件的RNA/DNA测序数据高通量分析的方法.

主要方法:

文献综述侧重于RNA编辑机制和癌症.
分析RNA编辑水平,ADAR和APOBEC基因表达,以及特定的编辑基因作为潜在的生物标志物.
探索高通量测序数据分析方法.

主要成果:

RNA编辑参数,包括编辑水平和ADAR和APOBEC基因的表达,显示出作为癌症生物标志物的希望.
特定的编辑基因可以作为癌症进展的指标.
目前的方法可以改进,以加速识别RNA编辑事件.

结论:

编辑RNA是癌症生物标志物发现的一个重要领域.
对ADAR和APOBEC的作用和特定编辑基因的进一步研究可以促进癌症诊断和预后.
优化高通量测序分析将促进RNA编辑生物标志物的临床应用.

关键词:

阿达尔 (ADAR) 是一个叫做ADAR的词.这是APOBECAPOBECAPOBECAPOBECAPOBECAPOBECAPOBECAPOBECAPOBECAPOBECAPOBECAPOBECAPOBEC Alu编辑索引编辑指数国家储备系统 (NGS)编辑RNA的RNA编辑有关RNA测序的RNA测序癌症进展癌症进展表观遗传学是指表观遗传学.标志性转录组学标志性转录组学可转移的元素可以转移.

更多相关视频

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Published on: July 22, 2014

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Published on: September 16, 2019

相关概念视频

RNA Editing

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

Exon Recombination

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...

Long-patch Base Excision Repair

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:

Base Excision Repair

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...

Proofreading

Proofreading

Mismatch Repair

Mismatch Repair