相关实验视频

Updated: May 9, 2025

Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation

Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation

Published on: July 6, 2019

巨细胞Notch1通过调节小鼠的CCR5表达方式参与LPS诱导的急性肺损伤

Ruiyu Zhang¹, Hui Du¹, Zhi Liu¹

¹Department of Pediatrics, Zhongnan Hospital of Wuhan University, 430071 Wuhan, Hubei, China.

Frontiers in bioscience (Landmark edition)

|April 30, 2025

概括

缺口信号通过控制C-C化学因受体5型 (CCR5) 表达来调节急性肺损伤 (ALI) 中的巨细胞透和炎症. 这种途径调节为ALI治疗提供了潜在的治疗点.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
肺部医学肺部医学

背景情况:

膜巨细胞 (AMs) 是肺部防御,炎症和修复的关键.
在急性肺损伤 (ALI) 期间,Notch通路在AMs中的作用需要进一步阐明.

研究的目的:

调查AMS中Notch通路激活如何影响ALI病变的发生.
为了确定Notch信号,C-C化学因受体5型 (CCR5) 和肺炎之间的调节关系.

主要方法:

使用脂聚糖 (LPS) 诱导了一种ALI模型.
研究使用了特定于巨细胞的Notch1淘汰赛小鼠和不朽化骨髓衍生巨细胞 (iBMDMs).
基因和蛋白质表达 (CCR5,炎症调解剂) 通过qRT-PCR和免疫光检测进行了分析;巨细胞迁移使用迁移试验进行了评估.

主要成果:

由LPS诱导的ALI显示了Notch细胞内域 (NICD) 和CCR5表达的增加.
与对照组相比,Notch1淘汰赛小鼠表现出较低的CCR5表达,较少的巨细胞透和较低的炎症前媒介转录 (p <0.05).
在Notch1缺乏的小鼠中,肺损伤的严重程度降低了 (p <0.05),而在体外,Notch抑制降低了CCR5表达和巨细胞迁移.

结论:

痕信号在LPS诱导的ALI期间调节巨细胞透中发挥着至关重要的作用.
诺奇通路调节CCR5的表达,影响ALI的炎症反应.
准痕信号可能代表了ALI的治疗策略.

关键词:

CCR5 CCR5 的意思是什么?没有口,没有口.肺部急性肺损伤是什么膜细胞巨细胞.细胞迁移细胞迁移

更多相关视频

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

Published on: March 21, 2021

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

相关概念视频

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...