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超高流量调节放电合与有针对性的质谱分析:实时捕获呼吸中的生物分子

Wenzhao Zhou¹, Wenting Wang², Mengmeng Jiang¹

¹Liangzhu Laboratory, Zhejiang University, Hangzhou, Zhejiang 311121, China.

Analytical chemistry

|April 30, 2025

概括

一种新的超高流量调节放电 (UFD) 电离方法允许通过质谱在线检测各种呼吸代谢物和宏分子. 这一进步使呼吸分析能够补充用于疾病诊断和代谢监测的血液测试.

科学领域:

分析化学分析化学
生物化学生物化学
代谢学代谢学代谢学

背景情况:

在线呼吸分析提供非侵入性疾病诊断和代谢监测.
现有的方法检测挥发性物质,通常与血液检测结果无关.
存在对检测呼吸中的非挥发性代谢物方法的需求.

研究的目的:

为在线呼吸分析提出和验证超高流量调节放电 (UFD) 电离方法.
为了能够在呼出的呼吸中检测到更广泛的生物活性代谢物和大分子.
评估呼吸分析作为血液检测的替代品的潜力.

主要方法:

开发了一种超高流量调节放电 (UFD) 电离技术,并与质谱学相结合.
在大气压下,冠状放电转化为发光放电,可能涉及声波喷雾机制.
对UFD与大气压化学电离 (APCI) 和电喷电离 (ESI) 进行覆盖,吞吐量和效率的比较.

主要成果:

UFD可以在线检测出口气中的81种生物分子,包括所有20种常见的氨基酸,核酸和三碳酸.
在呼吸中观察到63个以前未被检测到的成分.
检测到了巨分子,呼吸中的同类半氨酸水平与五个受试者的血液检测结果相关.

结论:

UFD方法显著扩大了可检测的呼吸代谢物的范围,包括非挥发性物质和大分子.
使用UFD进行在线呼吸分析显示,UFD可作为临床诊断的某些血液检测的非侵入性替代方案.
UFD为质谱学提供了一种通用的新接口,增强了分析复杂生物样本的能力.

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