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Updated: May 9, 2025

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完整的基因组序列和构建一个传染性细菌人工染色体克隆的病毒性肠炎病毒菌株XJ

Su-Xin Huo^1,2, Yin-Chu Zhu², Liu Chen²

¹College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.

Transboundary and emerging diseases

|April 30, 2025

概括

一种新的毒性肠炎病毒 (DEV) 菌株,DEV XJ,被测序,并创建了一个传染性克隆. 基因删除研究显示UL21和UL36对病毒增殖至关重要,而US3和LORF3则影响复制和传播.

科学领域:

病毒学病毒学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

一种高度毒性的肠炎病毒 (DEV) 菌株,DEV XJ,于2021年在中国分离出来.
DEV XJ与其他 DEV 菌株具有同质性,但具有独特的免疫性和毒性相关基因.

研究的目的:

为了测序DEV XJ菌株的完整基因组.
构建一个全长的传染性细菌人造染色体 (BAC) 克隆DEV XJ.
研究特定DEV基因的致病机制和功能作用.

主要方法:

关于DEV XJ的全基因组测序.
构建DEV XJ的感染性BAC克隆.
使用两步红色重组的US3,LORF3,UL21和UL36的基因删除.
病毒生长,复制和细胞间传播的体外分析.

主要成果:

对DEV XJ基因组进行了测序 (162,234 bp,78 ORF).
传染性XJ BAC克隆模仿了父母的DEV XJ在体外生长和致病性.
UL21和UL36基因对病毒增殖至关重要.
虽然US3和LORF3基因对复制不至关重要,但减弱了DEV复制和传播.

结论:

该研究成功地测序了一种毒性DEV菌株,并创建了一个感染性克隆.
UL21和UL36对于DEV的扩散至关重要.
US3和LORF3在调节DEV复制和传输效率方面发挥着重要作用.

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相关概念视频

Viral Recombination

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.