检测和鉴定致病性 Leptospira 菌种. 2017年至2020年期间在欧洲的血清组应用一种基于基因的新型分子方法
Jasmin Wenderlein1, Theresa Zitzl1, Nathalie Dufay-Simon2
1Chair for Bacteriology and Mycology Institute for Infectious Diseases and Zoonoses Department of Veterinary Sciences Faculty of Veterinary Medicine LMU Munich Sonnenstraße 24, Oberschleißheim85764Germany.
Transboundary and emerging diseases
|April 30, 2025
概括
在全球范围内,狗螺杆菌病正在重新出现. 这项研究确定了Leptospira Icterohaemorrhagiae和Leptospira Australis是欧洲狗中最常见的血清组,强调了当前L4疫苗的重要性.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 传染性疾病 传染性疾病
- 分子诊断学 分子诊断
背景情况:
- 瘦肉是一种重新出现的动物传染病,由于气候变化和城市化,欧洲的风险越来越大.
- 狗很容易受到Leptospira spp.的感染. 它们可以作为了解环境水库的哨兵动物.
- 研究病原性勒普托斯皮拉在狗中的患病率对于制定有效的保护措施,包括疫苗接种至关重要.
研究的目的:
- 为了选致病性Leptospira (L.) spp.的狗野外样本. 在欧洲. 在欧洲.
- 开发和应用一种新型的分子方法来识别Leptospiral血清组.
- 评估当前L4疫苗对流行血清组的相关性.
主要方法:
- 从239只狗的血液和尿液样本中分析了纯化的DNA.
- 一种新的分子方法涉及16S rRNA PCR和用于物种识别的测序.
- 随后针对rfb基因位点的PCR用于血清组分类.
主要成果:
- 在172个成功测试的样本中,Leptospira Icterohaemorrhagiae是最常见的血清组 (53%).
- 莱普托斯皮拉 (Leptospira Australis) 血清组是第二个最常见的 (13%).
- 其他确定的血清组包括L. Pomona (5%),L. Autumnalis (4%) 和L. Sejroe (2%).
结论:
- 该研究确定了欧洲狗中最常见的Leptospira血清组.
- 目前的L4疫苗可能对占主导地位的L. Icterohaemorrhagiae和L. Australis血清组有效.
- 这些发现支持L4疫苗的持续使用和相关性,以控制犬类白病.
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