相关实验视频

Updated: May 9, 2025

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

在中国分离的BJEU06-1-Like PRRSV-1 ZD-1的基因组特征和致病性

Hu Xu¹, Bangjun Gong¹, Qi Sun¹

¹State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.

Transboundary and emerging diseases

|April 30, 2025

概括

类似中国BJEU06-1的猪生殖和呼吸系统综合征病毒-1 (PRRSV-1) 菌株ZD-1在小猪中引起中度疾病. 这项研究为中国这一主要的PRRSV-1流行分支提供了第一个致病性数据.

科学领域:

兽医病毒学兽医病毒学
动物病理学动物病理学

背景情况:

猪生殖和呼吸系统综合征病毒-1 (PRRSV-1) 和PRRSV-2已经在中国共同传播.
中国的PRRSV-1菌株以前被归类为亚型1,BJEU06-1类菌株成为主要的流行病分支.
缺乏BJEU06-1类PRRSV-1亚组的致病性数据.

研究的目的:

为了隔离和表征一种类似于BJEU06-1的中国PRRSV-1菌株.
为了评估在小猪中隔离的菌株的致病性.
为了提高对中国PRRSV-1流行病学的理解.

主要方法:

从初级膜巨细胞 (PAMs) 中分离PRRSV-1菌株ZD-1.
对ZD-1的基因分析,注意到Nsp2中的5氨基酸缺失和GP3中的26氨基酸C终端切断.
猪仔感染ZD-1以评估临床症状,体重变化和各种组织中的病毒载荷.

主要成果:

该PRRSV-1株ZD-1表现出与BJEU06-1类似的分支的特征性遗传特征.
感染ZD-1的小猪长时间发烧,体重减轻,肺部病变 (膜上皮质增殖,膜扩张).
组织病理学揭示了淋巴结中的淋巴细胞增生,在血清,肺部,淋巴结和桃体中检测到高的病毒血症和病毒载荷.

结论:

类似中国BJEU06-1的PRRSV-1株ZD-1对小猪具有中等致病性.
这项研究提供了中国BJEU06-1类PRRSV-1分支的首次致病性评估.
这些发现有助于更好地了解PRRSV-1的多样性和该地区的影响.

更多相关视频

Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes

Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes

Published on: August 31, 2022

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

相关概念视频

Viral Mutations

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...

Viral Recombination

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.