致病性Leptospira sp. 的分子检测 在巴西海岸的鱼中
Felipe D'Azeredo Torres1, Ana Luiza Dos Santos Baptista Borges1, Pedro Volkmer de Castilho2
1Laboratory of Veterinary Bacteriology, Biomedical Institute, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil.
Transboundary and emerging diseases
|April 30, 2025
概括
在巴西14.8%的鱼中发现了致病性Leptospira细菌. 沿海物种的感染率更高,这表明海洋哺乳动物可以携带Leptospira,影响One Health.
科学领域:
- 海洋生物学 海洋生物学
- 动物传染病是动物传播的疾病.
- 微生物学 微生物学
背景情况:
- 牛螺杆菌病是一种由 * Leptospira * 螺杆菌引起的动物性疾病,在全球分布,特别是在热带和亚热带地区.
- 虽然在陆地动物和人类中得到了充分的证据,但在海洋动物,特别是类动物中,白病的发生和影响仍未得到充分的研究.
- 一种健康方法强调了人类,动物和环境健康的相互联系,需要对海洋哺乳动物等潜在的水库进行调查.
研究的目的:
- 为了调查病原性 *Leptospira* sp. 的存在. 在巴西海岸沿线的鱼中.
- 为了确定鱼是否可以作为Leptospira的载体,并了解海洋环境中的传播动态.
- 通过评估海洋哺乳动物在白病流行病学中的作用,为"一个健康"框架做出贡献.
主要方法:
- 收集和分析来自19个物种的142只鱼的脏样本.
- 用 *LipL32* - 聚合酶连锁反应 (PCR) 来检测致病性 *Leptospira*.的DNA提取,然后进行诊断.
- 通过*secY*基因测序和植物遗传学分析对阳性样本的遗传特征.
主要成果:
- 在14% (21/142) 的鱼中检测到致病性*Leptospira*DNA.
- 与海洋物种 (7.5%) 相比,沿海鱼物种的感染频率明显高 (25%).
- 测序的菌株被确定为*Leptospira interrogans*,与Icterohaemorrhagiae血清组密切相关,后代分析揭示了海洋哺乳动物中鲜明的循环类型.
结论:
- 类动物可以充当致病性 * Leptospira * * 的载体.
- 这项研究确定了在巴西海岸的海洋哺乳动物中循环的独特的 * Leptospira * 哈普洛类型.
- 靠近人为造成的地区可能会影响白病的传播动态,这强调了它在One Health环境中的相关性.
相关概念视频
Bacterial Phylum Spirochaetes
Spirochetes, unique bacteria in the phylum Spirochaetes, are gram-negative, motile, tightly coiled, slender, and flexible. They inhabit aquatic sediments and animals, with some causing diseases like syphilis. Spirochetes are classified into eight genera based on habitat, pathogenicity, phylogeny, and characteristics.Their distinctive motility arises from endoflagella, located within the cell’s periplasm. These endoflagella anchor at the cell poles and extend along the cell length, encased by a...
Leishmaniasis
Leishmaniasis is a protozoal disease caused by species of the genus Leishmania and transmitted through the bite of infected female sandflies. The parasite exists in two principal morphological forms during its life cycle. A sandfly acquires intracellular amastigotes from an infected reservoir host, such as a dog. Within the sandfly, these forms differentiate into motile, flagellated promastigotes. During a subsequent blood meal, promastigotes are injected into the human host, where they...


