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在黎巴嫩本地和进口牛群中对致病性Leptospira的分子和血清鉴定

Elena Harran^1,2, Alain Abi Rizk², Sophie Angelloz-Pessey³

¹VetAgro Sup, Université Claude Bernard Lyon 1, USC 1233, Marcy L'Etoile, Lyon, France.

Transboundary and emerging diseases

|April 30, 2025

概括

黎巴嫩的牛群中存在一种细菌性动物性疾病 - - 勒普托斯皮罗斯症,标记为*L. kirschneri*. 这项研究提供了黎巴嫩牛乳病的第一份流行病学数据,突出了农业和贸易中的风险.

科学领域:

兽医医学兽医医学兽医医学
动物传播疾病动物传播疾病
细菌学细菌学是一门学科.

背景情况:

螺杆菌病是一种重要的全球性细菌动物病,在温暖,潮湿的气候中患病率增加.
黎巴嫩的气候有利于Leptospira的持续存在,但缺乏人类和动物感染的正式预防系统.
在黎巴嫩,白病的流行病学仍然在很大程度上没有被描述,这引起了公众健康和经济方面的担忧.

研究的目的:

在黎巴嫩境内对牛群中 *Leptospira* 感染进行初步调查.
建立第一个关于黎巴嫩山省牛牛螺杆菌的流行病学数据和进口牛.
为了识别在黎巴嫩牛群中流通的致病性*Leptospira*物种和血清组.

主要方法:

一项涉及135个本地和52个进口牛血样和135个本地牛血清样本的描述性研究.
实时PCR向16SrRNA,用于血液中的初始 * Leptospira *检测.
常规PCR (cPCR) 和桑格测序用于物种识别,以及微观聚合试验 (MAT) 用于血清组的确定.

主要成果:

通过实时PCR检测,在135头本地牛中的7头和52头进口牛中的1头中检测到 *Leptospira* 感染.
测序在6只本地和1只进口受感染的动物中发现了*Leptospira kirschneri*.
在五个牛群中的七只本地牛中,MAT显示出显著的抗体标位 (≥1:100),其中血清组Grippotyphosa占主导地位.

结论:

这项研究提供了关于黎巴嫩牛 * Leptospira * 感染的第一个流行病学见解.
致病性 * Leptospira * 种 (* L. kirschneri *) 和血清组 (Grippotyphosa) 存在于本地和进口牛群中.
调查结果强调了黎巴嫩的牛感染风险,强调需要加强公共卫生意识和控制策略.

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