新发现的猪流行性腹病毒 GII 亚型 菌株
Jiarong Yu1, Pengfei Chen2, Ruilin Liu1
1Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
Transboundary and emerging diseases
|April 30, 2025
概括
猪流行性腹病毒 (PEDV) GII-a菌株在中国最为普遍. 这项研究确定了PEDV中独特的S蛋白突变和新的重组模式,这对于有效的疾病控制至关重要.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子流行病学分子流行病学
- 猪健康 猪健康 猪健康
背景情况:
- 猪流行性腹病毒 (PEDV) 由于腹爆发,在养猪场造成重大经济损失.
- 了解PEDV的遗传多样性和演变对于疾病监测和控制至关重要.
研究的目的:
- 分析在中国流传的PEDV GII菌株的S基因流行,变异和重组模式.
- 确定PEDV GII-a菌株S蛋白中的独特突变,并描述新的重组事件.
主要方法:
- 在两年内从临床样本中收集和分离了24个PEDV菌株.
- 对PEDV S基因的遗传学分析以确定进化关系.
- 对S蛋白的氨基酸突变分析.
- 选择PEDV分离物的全基因组测序.
- 重组分析以确定潜在的重组菌株.
主要成果:
- PEDV GII-a菌株是最常见的 (21/24个孤立),其次是GII-b (2/24个孤立).
- 在S蛋白中观察到显著的氨基酸突变 (43/常见),其中36个突变频率高于90%.
- 在GII-a菌株中发现了三个独特的氨基酸突变部位.
- 确定了一种新型重组菌株 (SD1),由外国S-INDEL和国内GII菌株产生的.
- 在过去的两年里,PEDV GII-a一直是中国流通的主要菌株.
结论:
- 在中国,PEDV GII-a亚型是流行的主要菌株.
- 在PEDV菌株中发生了独特的S蛋白突变和新的重组事件.
- 持续监测PEDV流行动态对于有效的预防和控制策略至关重要.
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