相关实验视频

Updated: May 9, 2025

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

鉴定了一种与牛呼吸道疾病相关的新型天体病毒

April Nelsen¹, David Knudsen^1,2, Ben M Hause^1,2

¹Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA.

Transboundary and emerging diseases

|April 30, 2025

概括

在患有呼吸道疾病的牛中发现了一种新的牛天文病毒 (BAstV). 这种病毒在肺组织中被检测到,这表明它可能在牛呼吸道感染中发挥作用,需要进一步调查.

科学领域:

兽医病毒学兽医病毒学
分子流行病学分子流行病学
传染性疾病传染性疾病

背景情况:

众所周知,天体病毒 (AstV) 在各种物种中引起胃肠道疾病.
越来越多的证据表明,AstVs可以感染肠外组织,包括呼吸道,并与疾病有关.
牛类天体病毒 (BAstV) 之前与牛的胃肠道或神经疾病有关.

研究的目的:

为了识别和表征一种新型的天体病毒,该病毒在来自呼吸系统疾病病例的牛肺样本中检测到.
在牛诊断样本中调查这种新型BAstV的流行率和组织热带性.

主要方法:

用转基因组测序来识别肺组织中的新型病毒.
使用定量逆转录PCR (qRT-PCR) 在肺部和肠道样本中检测BAstV.
进行RNAscope® in situ杂交 (ISH),以在肺组织中定位病毒核酸.
对病毒基因组进行了遗传和遗传学分析.

主要成果:

发现了一种新型的BAstV,其基因与山羊类天体病毒 (CAstV G2.1) 和先前发现的BAstV菌株 (BSRI-1) 有相似之处.
在8%的牛肺样本和2%的肠道样本中检测到新型BAstV.
在阳性肺样本中,病毒RNA主要存在于膜巨细胞和单核细胞的细胞质中.
遗传学分析显示,它与其他牛和山羊天体病毒有密切的关系.

结论:

在患有呼吸道疾病的牛中发现了一种新的牛天文病毒 (BAstV).
在肺组织中检测到BAstV表明它可能在牛呼吸道感染中发挥作用.
需要进一步的研究,以阐明牛呼吸系统疾病中BAstV的临床意义和病原性.

更多相关视频

Assays for the Identification of Novel Antivirals against Bluetongue Virus

Assays for the Identification of Novel Antivirals against Bluetongue Virus

Published on: October 11, 2013

The Bovine Lung in Biomedical Research: Visually Guided Bronchoscopy, Intrabronchial Inoculation and In Vivo Sampling Techniques

The Bovine Lung in Biomedical Research: Visually Guided Bronchoscopy, Intrabronchial Inoculation and In Vivo Sampling Techniques

Published on: July 3, 2014

相关概念视频

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...

Viral Recombination

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.