转录基因和蛋白质基因方法揭示了两种新型猪原型非编码DNA分子的生物学功能
Libin Wen1,2,3,4,5, Jianping Xie1,2,3,4,5, Qi Xiao1,2,3,4,5
1Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Transboundary and emerging diseases
|April 30, 2025
概括
猪圈病毒样微型剂 (PCVLs) 是具有未知的生物活性的小圆形DNA分子. 这项研究使用了转录组和蛋白组分析来揭示PCVL258和PCVL264感染对小鼠的代谢,癌症和神经退行性途径的影响.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 在动物中发现了小型圆形非编码DNA分子,称为猪肉环状病毒类微型代理物 (PCVLs).
- PCVLs的生物功能和病原性潜力尚未被理解.
研究的目的:
- 通过分析它们对宿主基因和蛋白质表达的影响来研究PCVLs的生物活动.
- 为了比较两个不同的PCVL分子克隆PCVL258和PCVL264.4的分子影响.
主要方法:
- 对感染PCVL258或PCVL264分子克隆的BALB/c小鼠的肝脏组织进行了转录和蛋白质组分析.
- 不同的基因和蛋白质表达在感染后7天和28天 (dpi) 被评估.
- 基因本体学和基因和基因组的京都百科全书 (KEGG) 途径分析被用来解释数据.
主要成果:
- PCVL258感染导致了基因和蛋白质的差异表达,这些基因和蛋白质主要丰富于代谢,癌症和神经退行性疾病途径.
- PCVL264感染导致了差异性的基因和蛋白质表达,主要与代谢,生殖和胰腺途径有关.
- 这项研究代表了第一个结合的转录和蛋白质学方法,以阐明小病原体相关的DNA分子的生物活动.
结论:
- 转录基因和蛋白质基因分析揭示了与不同PCVL分子克隆相关的独特生物活动.
- PCVL感染可以显著改变宿主细胞通路,包括与新陈代谢,癌症和神经退行相关的.
- 了解PCVLs的宿主-病原体相互作用对于评估它们在疾病产生中的作用至关重要.
相关概念视频
Complementary DNA
Overview
piRNA - Piwi-interacting RNAs
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...


