从狗身上分离出来的猫类病毒的遗传进化和生物学特征
Fanyuan Sun1, Xinyi Guo1, Jinfan Guo1
1Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Transboundary and emerging diseases
|April 30, 2025
概括
猫病毒 (FCV) 已在狗中检测到,证实了物种间的传播. 这项研究分析了犬类FCV菌株,揭示了遗传多样性和重组,影响了我们对FCV进化和犬类健康的理解.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子流行病学分子流行病学
- 传染性疾病 传染性疾病
背景情况:
- 猫病毒 (FCV) 是一种传染性病原体,导致猫的口腔和呼吸系统疾病,具有潜在的肠道作用.
- 对于犬类FCV的流行病学和生物特征的了解有限,尽管在犬类肠道中发现了一些类似FCV的病毒.
研究的目的:
- 为了调查猫病毒 (FCV) 在狗中的存在,遗传多样性和生物特性.
- 确认猫和狗之间的FCV跨物种传播.
主要方法:
- 在2020-2021年期间,从狗身上收集和分析了252个便样本.
- 隔离和完整的基因组测序犬起源的FCV菌株.
- 遗传学分析,对VP1蛋白质的同一性比较,以及重组分析.
- 在MDCK细胞中的复制试验和斑块表型比较.
- 使用接种疫苗的猫和狗血清进行中和抗体测试.
主要成果:
- 在狗中发现了六个FCV阳性样本 (2.41%);两个FCV菌株被分离并测序.
- 遗传学分析将犬类FCV分离物置于基因组I内,显示出基因多样性和在一个分离物中与猫类菌株重组的证据.
- 狗类FCV分离物在MDCK细胞中复制效率低下,但表现出与呼吸道FCV菌株相似的斑块表型.
- 疫苗接种猫的血清观察到低交叉反应性,而对犬类FCV的高中和抗体标位在狗血清中发现,证实了物种间传播.
结论:
- 猫类卡利西病毒 (FCV) 在狗群中传播,有证据表明从猫间的跨物种传播.
- 狗的FCV分离物表现出遗传多样性和重组,有助于理解FCV演变.
- 这些发现强调了对猫和狗群体的FCV监测对于有效的疾病控制的重要性.
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