对Listeria monocytogenes病毒性计划的压力依赖激活确保了细菌在感染期间的耐药性
Mariya Lobanovska1, Ying Feng1, Jonathan Zhang1
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.
mBio
|April 30, 2025
概括
细菌应激酶对Listeria monocytogenes至关重要,以保持种群的一致性,并在宿主感染期间激活毒性. 这种信号机制确保了对病原体生存的强有力的应激反应.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 细胞微生物学 细胞微生物学
背景情况:
- 李斯特菌 (Listeria monocytogenes) (Lm) 使用家庭 (P1) 和压力激活 (P2) 促销者进行病毒性基因表达.
- 包含300多个基因的Sigma B regulon对各种压力因素作出反应,但其在感染期间调节prfA毒性基因的作用尚不清楚.
研究的目的:
- 确定调节Lm中的Sigma B-依赖prfA激活的途径.
- 调查压力酶在Lm病毒性和感染期间人口均质性中的作用.
主要方法:
- 在L2纤维细胞中使用ΔP1 Lm菌株进行了基因选,以确定参与Sigma B-依赖prfA激活的基因.
- 转子体突变发生被用来选影响病毒性突变的突变.
主要成果:
- 屏幕确定了压力酶,一种细菌感官器官,对于Sigma B-依赖的prfA激活至关重要.
- 应激素组分的突变导致了种群异质性,其中存在真空逃逸和/或细胞间传播的缺陷.
- 这种异质性无法通过构成性PrfA激活来挽救,突出强调了压激素在同质性中的作用.
结论:
- 压力酶对于保持细菌群体的一致性和确保感染期间L. monocytogenes中强大的毒性激活至关重要.
- 压力酶感知宿主施加的压力,将压力反应与毒性基因调节联系起来.
- 建立了一个 ΔP1 Lm 模型来研究 L. monocytogenes 病原体的压缩体依赖信号和宿主特异信号.
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