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评估MicroScan和VITEK 2系统,用于对肠杆菌的敏感性测试,并更新了断点

Sandra S Richter¹, Elizabeth L Dominguez¹, Aaron A Hupp¹

¹Department of Laboratory Medicine and Pathology, Mayo Clinic, Jacksonville, Florida, USA.

Journal of clinical microbiology

|April 30, 2025

概括

两种商业抗微生物敏感性测试系统对肠杆菌表现出类似的准确性,MicroScan需要更少的实践时间,VITEK提供更快的结果. 这两种系统都可靠地检测抗菌素耐药性.

科学领域:

临床微生物学临床微生物学
抗微生物药物耐药性的监测监测
诊断测试评价诊断测试评价诊断测试评价

背景情况:

准确的抗微生物敏感性测试 (AST) 对于指导患者治疗和监测耐药性趋势至关重要.
商业AST系统在临床实验室中广泛使用,但其性能需要与参考方法进行持续评估.
监管机构更新的断点需要对AST系统进行验证,以确保结果的准确解释.

研究的目的:

为了比较两种商业AST系统的性能,VITEK 2和MicroScan,与当代Enterobacterales分离物的微稀释 (BMD) 相比.
为了评估两个系统的基本协议 (EA),分类协议 (CA),错误率,动手时间和结果时间 (TTR).
评估这些系统在检测抗菌素耐药性的实用性,使用当前的2022临床和实验室标准研究所 (CLSI) 或FDA断点.

主要方法:

同时使用VITEK 2 AST卡和MicroScan NM56面板对肠细菌临床分离物 (n=200) 进行了测试.
调料微稀释 (BMD) 作为参考标准,结果使用2022 CLSI/FDA断点进行解释.
计算和比较了基本协议 (EA),分类协议 (CA),错误率,实践时间和仪器TTR.

主要成果:

无论是VITEK 2还是MicroScan都表现出高准确度,总体CA> 94%和EA> 96%.
对于大多数抗微生物药物,分类一致性为≥90%,但对于安培-苏尔巴克坦,塞福西丁,米诺环素和尼托福兰来说,有例外.
与VITEK 2 (1.83分钟/隔离) 相比,MicroScan需要更少的手动设置时间 (1.29分钟/隔离),而VITEK 2则提供了显著更快的中位仪器TTR (11.7小时与18小时相比).

结论:

维特克2和MicroScan都是准确的商业AST系统,适用于当前断点的临床使用.
在选择AST系统时,实验室应考虑实践时间和周转时间之间的权衡.
这些发现支持更新断点的验证和实施,以确保可靠地检测抗菌素耐药性.

关键词:

抗微生物药物是一种抗微生物药物.易感性测试易感性测试易感性测试

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