揭示了精子功能障碍中的piwi相互作用RNA和类固醇5α减少酶2型之间的分子交叉对话
Adnan Fadhel Al-Azaawie1, Ahmed AbdulJabbar Suleiman2, Mousa Jasim Mohammed1
1Department of Biology, College of Science, University of Tikrit, Tikrit, Iraq.
F&S science
|April 30, 2025
概括
改变的piwi相互作用RNA (piRNA) 配置文件和类固醇5α减少酶2型 (SRD5A2) mRNA表达与男性不孕症相关. 这些发现表明piRNAs可能调节精子生成,并作为男性不孕不育的生物标志物.
科学领域:
- 生殖生物学和分子遗传学.
背景情况:
- 男性不孕症影响了试图怀孕的夫妇的很大一部分.
- 类固醇5α减少酶2型 (SRD5A2) 在雄激素代谢中起着至关重要的作用,影响男性生殖功能.
- 皮维相互作用RNAs (piRNAs) 正在成为生殖线发育和基因沉默的关键调节者.
研究的目的:
- 为了研究piRNA表达和精液中SRD5A2mRNA调节之间的关系.
- 探索不同男性不孕不育亚型的潜在相关性:精,精和精.
主要方法:
- 来自88名男性参与者的精液分析 (不孕症和常态精子组).
- 使用qRT-PCR对SRD5A2mRNA和特定piRNAs (hsa-piR-002528,hsa-piR-017183,hsa-piR-023244,hsa-piR-023338) 的量化进行了测量.
- 相关性分析以评估piRNA水平和SRD5A2表达之间的相互作用.
主要成果:
- 在不孕症亚型中观察到精子参数的显著差异.
- 在所有不孕症组中,SRD5A2 mRNA的表达发生了变化,在精中呈上调,在橄精和精中呈下调.
- 特定的piRNAs (hsa-piR-002528,hsa-piR-017183) 在所有不孕症类型中都被上调,而其他类型则显示了亚型特定的模式.
- 在SRD5A2mRNA和hsa-piR-002528/hsa-piR-023338之间存在负相关性,这表明它们在精子功能的调节作用.
结论:
- 不同的piRNA配置文件和SRD5A2表达模式与男性不孕症有关.
- 这些发现强调了piRNAs在精子生成中的调节作用.
- piRNAs和SRD5A2代表了男性不孕症的潜在生物标志物和治疗点.
相关概念视频
piRNA - Piwi-interacting RNAs
6.7K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.7K
RNA Interference
25.7K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
25.7K
Experimental RNAi
6.0K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.0K
Infertility in Males
153
Male infertility affects millions of couples worldwide, arising from various factors that impact different stages of the reproductive process. An endocrine imbalance resulting from conditions like hypogonadism, Klinefelter syndrome, or pituitary disorders can disrupt hormone levels and reduce sperm production. Testicular defects, such as tumors, cryptorchidism, atrophic testes, abnormal sperm morphology, and low sperm count or motility, may arise due to genetic factors, structural...
153
MicroRNAs
2.9K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
2.9K
Spermatogenesis
101.7K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
101.7K


