红三叶草异黄素对乳母牛的反动物微生物组成和发酵的作用
Ying Bu1, Xiaoyin Zhang1, Zhanbo Xiong1
1State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Applied microbiology and biotechnology
|April 30, 2025
概括
红三叶草的异黄素,特别是生物素A,降低了乳氨和尿酶活性. 这提高了的利用率,并促进了奶牛的有益细菌.
科学领域:
- 体微生物学和发酵过程
- 动物营养和料效率动物营养和料效率
- 在反动物中的植物二次代谢产物.
背景情况:
- 众所周知,红三叶草异黄 (生物素A,formonetin) 可以提高反动物的料效率和利用率.
- 它们对乳头发酵动力学和微生物生态学的确切影响需要进一步阐明.
研究的目的:
- 为了研究红三叶草异黄对乳头发酵参数,酶活性和奶牛中的细菌多样性的影响.
- 评估生物素A和莫诺尼丁对尿素水解的体外影响.
主要方法:
- 在40只荷尔斯坦乳牛接受不同剂量的红三叶草异黄的体内研究.
- 试验室乳头发酵试验评估生物素A和甲对尿素分解的影响.
- 用于细菌组成分析的枪支元基因组测序.
- 测量液氨和尿酸酶活性的测量.
主要成果:
- 红三叶草异黄素显著降低了乳氨和尿酶活性 (P < 0.05).
- 甲基因组分析显示蛋白质和尿素分解细菌的丰富性下降,细胞分解细菌的增加 (P < 0.05).
- 实验室试验证实,生物素A和甲素减少了尿素的分解,而生物素A显示出更强的效果.
结论:
- 生物素A被确定为红三叶草中主要活性异黄素,负责调节尿素溶解和小麦芽发酵.
- 红三叶草异黄有效减少尿解细菌和氨产量,提高利用率.
- 这些发现支持使用红三叶草异黄来改善乳牛的功能和效率.
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