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Glycopeptide Capture for Cell Surface Proteomics
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优化生物化检测方法用于表征细胞表面蛋白质组.

Tamás Langó1, Katalin Kuffa1,2, Gábor E Tusnády3

  • 1Protein Bioinformatics Research Group, Institute of Molecular Life Sciences, Research Centre for Natural Sciences, Hungarian Research Network, Budapest, Hungary.

Methods in molecular biology (Clifton, N.J.)
|April 30, 2025
PubMed
概括

本研究引入了一种使用生物化进行细胞表面蛋白 (CSP) 分析的高通量方法. 这种技术可以有效地识别多种CSP特异性,用于各种分子生物学应用.

关键词:
生物化类.细胞表面可访问的蛋白质区域.膜蛋白是一种膜蛋白.纳米HPLC的分离方法类序列的测序方法一些表面的表面.协奏式质谱测量 质谱测量

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.