相关实验视频
Updated: May 9, 2025

06:59
Nanomanipulation of Single RNA Molecules by Optical Tweezers
Published on: August 20, 2014
14.7K
基于结构的原则,这些原理是基础的,用于对丁-II рибо开关的连接体识别
Xiaochen Xu1,2, Mengqi He1, Xiaoqing Tai1
1Department of Cardiology, Second Affiliated Hospital of Zhejiang University School of Medicine, Life Sciences Institute, Zhejiang University, Hangzhou, 310058, China.
Science China. Life sciences
|April 30, 2025
概括
研究人员阐明了与氨酸结合的氨酸-II рибо开关的结构,揭示了一个独特的结合口袋. 这一发现使得开发一种新型生物传感器能够检测丁,有助于诊断代谢障碍.
科学领域:
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
- 结构生物学 结构生物学
背景情况:
- рибо开关是调节基因表达以应对特定代谢物的RNA分子.
- 已知有两种类型的对氨酸有反应的 рибо开关,对于维持氨酸平衡至关重要.
- 克桑丁-II рибо开关是由关氨酸 рибо开关衍生而来的,具有独特的结构修改.
研究的目的:
- 为了确定与丁结合的丁-II 杆切换器的复杂结构.
- 了解丁识别和特异性的分子基础.
- 探索丁-II рибо开关在开发丁生物传感器中的应用.
主要方法:
- 进行X射线晶体学以确定三维结构的Xanthine-II riboswitch-xanthine复合体.
- 异热定位热量计 (ITC) 通过基于结构的突变来验证联结特异性.
- 让丁-II рибо开关与Pepper原性吸体的融合,以创建一个生物传感器.
主要成果:
- 克桑-II рибо开关采用了三向连接结构,类似于瓜 рибо开关.
- 一个独特的结合口袋,由特定的突变和核酸插入形成,赋予高丁特异性.
- 赞丁与C64和G37形成一个基三重体,并且通过基配对和基三重体进一步稳定.
- 结合的测定证实了丁-II рибо开关的特异性.
- 成功开发了一种灵敏和特定的丁生物传感器.
结论:
- 克桑-II рибо开关的结构阐明揭示了克桑结合的独特机制.
- 独特的结构特征使其具有很高的特异性,使其与关氨酸 рибо开关区分开来.
- 赞丁-II рибо开关具有重要的潜力,用于开发用于赞丁代谢障碍的诊断工具.
相关概念视频
Riboswitches
8.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K
Types of RNA
62.6K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
62.6K
Ligand Binding and Linkage
4.7K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.7K
Ligand Binding Sites
12.6K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
12.6K
Allosteric Proteins-ATCase
5.6K
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.6K
Conserved Binding Sites
4.1K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.1K

