生物信息学分析揭示了与脊椎间盘退化中的编程细胞死亡相关的枢纽基因
Mingyang Zou1, Shaobo Wu1, Jundan Wang1
1Department of Orthopaedics, Lanzhou University Second Hospital, Lanzhou, 730030, Gansu, China.
Applied biochemistry and biotechnology
|April 30, 2025
概括
椎间盘退化 (IVDD) 涉及多种编程细胞死亡 (PCD) 途径. 研究人员确定YWHAB,BID和GSDME是IVDD的关键基因,开发了一个更好的管理的预后模型.
科学领域:
- 生物医学科学 生物医学科学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 椎间盘退化 (IVDD) 是一种慢性疾病,以编程细胞死亡 (PCD) 途径为标志.
- 在IVDD中识别与细胞死亡相关的基因对于诊断和预后至关重要.
研究的目的:
- 确定与IVDD细胞死亡相关的关键基因和途径.
- 根据细胞死亡生物标志物开发IVDD的预后模型.
主要方法:
- 分析了来自正常和IVDD标本的转录组数据.
- 使用了基因组变异分析 (GSVA) 和权重基因共同表达网络分析 (WGCNA).
- 使用LASSO回归和确定基因标构建了一个预后模型.
主要成果:
- 特定的PCD途径,包括亡,铁亡和自,在IVDD中被上调.
- 免疫分析显示IVDD组织中M0巨细胞增加.
- 确定了YWHAB,BID和GSDME作为IVDD病变发生的关键基因.
结论:
- 建立了一个整合IVDD和PCD机制的新框架.
- 作为IVDD的诊断生物标志物和治疗点,YWHAB,BID和GSDME显示出有前途.
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