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开发一种优化的两步固相提取方法,用于尿核酸导管学.

Alexandra Keidel1,2, Jazmine Virzi1, Laura Deloso1

  • 1Oxidative Stress Group, Department of Molecular Biosciences, University of South Florida, Tampa, FL 33620, USA.

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概括
此摘要是机器生成的。

这项研究开发了一种新的方法来提取和测量尿液中的核酸添加物,这对于了解疾病病因至关重要. 优化的固体相提取技术在人类和小鼠样本中成功识别了数百个 adducts.

关键词:
在DNA中,添加物是DNA的添加物.造成的DNA损伤是DNA损伤.修复DNA的修复DNA的修复引进的RNA是RNA的添加物.添加组学 添加组学 添加组学显露体 显露体 显露体质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量尿液 尿液 尿液

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科学领域:

  • 环境健康科学 环境健康科学
  • 毒理学 毒理学 毒理学
  • 分析化学 分析化学

背景情况:

  • 暴露组包括所有影响健康的暴露.
  • 通过暴露形成的核酸添加物与癌症等疾病有关.
  • 尿液分析提供了一种非侵入性的方法来研究身体的 adductome.

研究的目的:

  • 开发一种优化的固相提取 (SPE) 方法,以最大限度地从尿液中回收各种核酸添加物.
  • 通过非向的高分辨率质谱测试,证明该方法在识别内源性尿 adducts 的有效性.

主要方法:

  • 评估各种SPE吸附剂组合用于从尿液中提取核酸添加物.
  • 使用ENV+和PHE SPE列组合来增强 adduct 保留.
  • 使用非定位的高分辨率质谱与FeatureHunter 1.3软件用于引证识别.

主要成果:

  • 在ENV+和PHE SPE组合中,表现出优异的恢复效果,相比于20个添加剂的标准尾酒.
  • 该方法成功地从老鼠和人类尿液中回收了内源核酸添加物.
  • 在这两种物种中确定了大约500种附加物,具有同位素标记的标准,有助于内生附加物表征.

结论:

  • 一种优化的SPE方法与高分辨率质谱学相结合,可以对尿中核酸添加体进行全面分析.
  • 这种方法有助于研究与暴露相关的健康影响和疾病机制.
  • 这种特征的附带体为了解个体的易感性和疾病风险提供了基础.