一种独特的IGHV3-66SARS-CoV-2中和抗体,由Omicron变种的初级感染引起
Qing Fan1, Congcong Liu1, Huimin Guo1
1The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, Shenzhen, Guangdong Province 518112, China.
一个新的单克隆抗体,ConBA-998,从一个主要的Omicron BA.1感染中被识别出来. 这种抗体向SARS-CoV-2尖端蛋白,提供了对Omicron特定抗体反应的见解.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 结构生物学 结构生物学
背景情况:
- SARS-CoV-2 Omicron 亚型变种迅速发展,逃避中和抗体 (nAbs).
- IGHV3-53/3-66公共nAbs显示了中和SARS-CoV-2的潜力.
- 通过原发性Omicron感染诱导IGHV3-53/3-66nAbs以前是未知的.
研究的目的:
- 为了调查原发性Omicron感染是否会引起IGHV3-53/3-66 nAbs.
- 为了描述由Omicron BA.1感染引起的新型单克隆的nAb.
主要方法:
- 从一个感染BA.1的个体中分离和描述单克隆的nAb (ConBA-998).
- 绑定亲和度测试和S1子单位脱落测试.
- 低温电子显微镜 (cryo-EM) 用于确定抗体-尖峰相互作用的结构基础.
主要成果:
- 鉴定了ConBA-998,一种IGHV3-66编码的单克隆NAB,由初级BA.1感染引起.
- ConBA-998表现出对Omicron依赖的中和和高结合亲和力.
- 克里奥-EM揭示了ConBA-998与Omicron BA.1尖端蛋白的受体结合域 (RBD) 的独特结合方式.
结论:
- 奥米克朗感染可以引起独特的nAbs,与奥米克朗前变种诱导的不同.
- ConBA-998的独特结合模式为SARS-CoV-2变异特异性抗体反应提供了洞察力.
- 这些发现有助于理解抗体逃避和开发针对性治疗方法来对抗不断演变的SARS-CoV-2变种.
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