短的神经F受体调节了Hyphantria cunea中的食和繁殖
Lili Sun1, Chunmiao Sun1, Jingjing Yin1
1Key Laboratory of Sustainable Forest Ecosystem Management-Ministry of Education, School of Forestry, Northeast Forestry University, Harbin, China.
International journal of biological macromolecules
|April 30, 2025
概括
在Hyphantria cunea中,神经F受体 (sNPFRs) 调节养,生长和繁殖. 沉默这些受体会损害昆虫的生长,生育能力和卵子生产,这表明它们是害虫控制的关键目标.
科学领域:
- 昆虫生理学 昆虫生理学
- 分子生物学分子生物学
- 神经内分泌学神经内分泌学
背景情况:
- 神经是神经系统中至关重要的信号分子.
- 短性神经F受体 (sNPFRs) 在生理调节中发挥作用.
- 了解像Hyphantria cunea这样的昆虫中的sNPFR功能对于害虫管理至关重要.
研究的目的:
- 在Hyphantria cunea.ea中识别和描述sNPFRs.
- 研究sNPFRs在生长,养和繁殖中的作用.
- 探索sNPFRs作为昆虫控制战略的目标的潜力.
主要方法:
- 对sNPFR基因 (sNPFR-A10和sNPFR-A11) 的识别和表达分析.
- 在幼虫和中使用小干扰RNA (siRNA) 进行基因沉默.
- 评估养行为,生长速度和代谢参数.
- 评估生殖参数,包括卵子生产,化能力和精子形态.
- 分子对接和AlphaFold分析用于预测受体-连接体相互作用.
主要成果:
- sNPFR-A10和sNPFR-A11在发育过程中和在特定的组织 (中肠,马尔比吉管,丸) 中表现出不同的表达模式.
- 沉默sNPFRs降低了食物摄入量,生长速度,并改变了幼虫的葡萄糖和甘油三水平.
- 生殖功能显著受损,卵子产量减少,化能力降低,幼的精子形成和运动受到干扰.
- 分子对接证实了sNPF作为sNPFRs的配体.
结论:
- sNPFRs是Hyphantria cunea中食,生长和生殖生理学的重要调节者.
- 这些受体是神经网的组成部分,影响多种生理过程.
- sNPFRs代表了开发针对H. cunea. 新型害虫控制策略的有希望的分子标.
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