siRNA纳米颗粒的配方,转染和增强的粘附 - 透到鼻子粘膜组织中
Leslie Vanessa Sanchez-Castillo1, Fabiola Guareschi2, Eleni Tsekoura1
1Department of Chemical and Materials Engineering, Faculty of Engineering, University of Alberta, 116 St & 85 Avenue, Edmonton, AB T6G 2G6, Canada.
概括
三甲基基托 (TMC) 纳米颗粒有效地提供基因治疗剂,如用于EGFR基因沉默的小干扰RNA (siRNA). 这些纳米颗粒显示出有希望的粘膜粘合和粘膜透,用于非侵入性输送.
科学领域:
- 生物技术是生物技术.
- 纳米医学是一种纳米医学.
- 基因治疗 基因治疗
背景情况:
- 基因疗法对治疗疾病具有前景,但有效的传递系统至关重要.
- 小干扰RNA (siRNA) 为基因沉默提供了一个有针对性的方法.
- 为siRNA开发安全高效的输送载体是纳米医学的一个关键挑战.
研究的目的:
- 为了评估三甲基基托 (TMC) 纳米颗粒用于小干扰RNA (siRNA) 输送.
- 为了优化TMC纳米粒子配方的复杂化,释放和转化效率.
- 评估TMC纳米颗粒的粘膜粘合和粘膜透特性,以进行非侵入性输送.
主要方法:
- 在各种siRNA:TMC重量对重量比率下制备TMC纳米粒子.
- 结合效率的评估,释放特征 (用肝素),物理性质和细胞毒性.
- 在HeLa细胞中对EGFR敲击效率的评估.
- 探索添加剂 (德克斯特兰硫酸盐,三聚酸盐,氨酸) 以提高性能.
- 在子鼻腔粘膜上使用双光子显微镜进行粘结和粘膜透研究.
主要成果:
- 较高的TMC比率增强了siRNA结合,降低了药物释放率.
- 添加剂提高了纳米粒子的稳定性.
- 优化的配方表现出高细胞活力和显著的EGFR基因沉默.
- 与自由siRNA相比,TMC纳米颗粒显示出优异的粘膜粘合和粘膜透.
结论:
- TMC纳米粒子是siRNA传递的有效载体,可以实现显著的EGFR基因沉默.
- 配方优化和添加剂的使用提高了纳米粒子的性能.
- 由于其粘粘性和粘膜透性特性,TMC纳米颗粒显示出非侵入性基因疗法应用的潜力.
关键词:
基因沉默是一种基因沉默.粘液凝聚力 粘液凝聚力粘膜透 粘膜透是一种鼻腔传递是通过鼻子进行的.小的干扰RNA可以干扰RNA.三甲基基三甲基基三甲基基三甲基基三甲基基三甲基基三甲基基三甲基基三甲基基三甲基基三甲基三甲基基三甲基基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基基三甲基基三甲基基三甲基三甲基三甲基三甲基基三甲基基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲基三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲三甲四更多相关视频
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