Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Abnormal Proliferation02:23

Abnormal Proliferation

4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.1K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.1K
Epigenetic Regulation01:37

Epigenetic Regulation

3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.0K
Negative Regulator Molecules01:23

Negative Regulator Molecules

35.2K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.2K
RNA Stability01:53

RNA Stability

33.4K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.4K
MicroRNAs01:22

MicroRNAs

21.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Magrolimab Plus Azacitidine Versus Placebo Plus Azacitidine in Patients With Untreated Higher-Risk Myelodysplastic Syndromes: The Phase III ENHANCE Study.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2026
Same author

Durable response to salvage gilteritinib, allogeneic stem cell rescue and maintenance therapy in relapsed AML with a <i>FLT3</i> N676K variant.

Leukemia & lymphoma·2026
Same author

Real-world, multi-omics validation of the clinical relevance of molecular taxonomy for myelodysplastic syndromes (MDS).

HemaSphere·2026
Same author

ELN-DAVID Recommendations for NGS-based FLT3-ITD MRD Testing in Patients with Acute Myeloid Leukemia.

Blood·2026
Same author

The roles of the acetyltransferase domains of the chromatin regulators KAT6A and KAT6B in vivo.

Development (Cambridge, England)·2026
Same author

Olutasidenib as maintenance therapy after treatment response in acute myeloid leukemia with persistent <i>IDH1</i> mutation.

Blood neoplasia·2026

相关实验视频

Updated: Jun 14, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

9.5K

转录组变化包括p53调节失调的主要DNMT3A突变细胞用于转化.

Erin M Lawrence1,2, Amali Cooray1,2, Andrew J Kueh1,2,3,4

  • 1Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.

EMBO reports
|April 30, 2025
PubMed
概括

DNMT3A突变通过损害血液细胞干细胞中的DNA甲基化和p53信号,加速小鼠淋巴瘤的发展. 这导致自我更新的增加和恶性转变的更高风险.

关键词:
基因甲基化 DNA 甲基化在DNMT3A中,它是DNMT3A.表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.基因工程是一种基因工程.血液学癌症 血液学癌症

更多相关视频

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

9.7K
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

2.5K

相关实验视频

Last Updated: Jun 14, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

9.5K
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

9.7K
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

2.5K

科学领域:

  • 遗传学 是一个遗传学.
  • 癌症生物学 癌症生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 在血液癌症中,DNA甲基转移酶3A (DNMT3A) 突变很常见.
  • DNMT3A中的R882H突变是人类恶性瘤中已知的"热点".
  • 了解DNMT3A突变的功能影响对于开发向疗法至关重要.

研究的目的:

  • 在小鼠模型中研究DNMT3A突变 (R878H) 的功能后果.
  • 探索DNMT3A突变在造血干/原生细胞 (HSPC) 行为和辐射敏感性中的作用.
  • 阐明DNMT3A驱动型白血病发生的分子机制.

主要方法:

  • 在Dnmt3a基因中生成了一种异构R878H突变的小鼠模型.
  • 使用Lin-Sca-1+Kit+ (LSK) HSPCs进行了竞争性移植试验.
  • 利用RNA测序来分析辐射LSK细胞中的基因表达变化.
  • 通过流细胞计量评估p53通路激活和PUMA表达.

主要成果:

  • R878H突变导致了全球DNA低甲基化,并在暴露于玛辐射后加速了胸腺淋巴瘤的发展.
  • Dnmt3aR878H/+ LSK HSPC表现出比野生类型对应物增强的自我更新能力.
  • 照射Dnmt3aR878H/+的LSK细胞显示p53通路下调和减少PUMA表达.
  • 在被辐射的突变小鼠的骨髓中证实了受损的p53信号传递.

结论:

  • DNMT3A R878H突变为HSPC提供了竞争优势,促进了以差异化为代价的自我更新.
  • 这些突变通过损害p53反应,使细胞对基因毒性压力敏感.
  • 由DNMT3A突变驱动的LSK细胞中的微妙的转录基因改变有助于白血病发生.